IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v3y2012i1d10.1038_ncomms1941.html
   My bibliography  Save this article

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

Author

Listed:
  • Zhaojun Ding

    (Ghent University
    The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University)

  • Bangjun Wang

    (Plant Biology, University of Fribourg)

  • Ignacio Moreno

    (Center for Genome Regulation, Center of Plant Biotechnology, Facultad de Ciencias Biologicas, Universidad Andres Bello)

  • Nikoleta Dupláková

    (Institute of Experimental Botany ASCR)

  • Sibu Simon

    (Ghent University)

  • Nicola Carraro

    (Purdue University)

  • Jesica Reemmer

    (Purdue University)

  • Aleš Pěnčík

    (Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University)

  • Xu Chen

    (Ghent University)

  • Ricardo Tejos

    (Ghent University)

  • Petr Skůpa

    (Institute of Experimental Botany ASCR)

  • Stephan Pollmann

    (Ruhr-Universität Bochum, Lehrstuhl für Pflanzenphysiologie)

  • Jozef Mravec

    (Ghent University)

  • Jan Petrášek

    (Institute of Experimental Botany ASCR)

  • Eva Zažímalová

    (Institute of Experimental Botany ASCR)

  • David Honys

    (Institute of Experimental Botany ASCR)

  • Jakub Rolčík

    (Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University)

  • Angus Murphy

    (Purdue University)

  • Ariel Orellana

    (Center for Genome Regulation, Center of Plant Biotechnology, Facultad de Ciencias Biologicas, Universidad Andres Bello)

  • Markus Geisler

    (Plant Biology, University of Fribourg)

  • Jiří Friml

    (Ghent University
    Faculty of Science, and CEITEC, Masaryk University)

Abstract

Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.

Suggested Citation

  • Zhaojun Ding & Bangjun Wang & Ignacio Moreno & Nikoleta Dupláková & Sibu Simon & Nicola Carraro & Jesica Reemmer & Aleš Pěnčík & Xu Chen & Ricardo Tejos & Petr Skůpa & Stephan Pollmann & Jozef Mravec , 2012. "ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis," Nature Communications, Nature, vol. 3(1), pages 1-11, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1941
    DOI: 10.1038/ncomms1941
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms1941
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms1941?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Huaiguo Shang & Na Zhang & Zhouli Xie & Siyu Deng & Licong Yi & Xing Huang, 2021. "Genome-Wide Identification and Expression of the PIN Auxin Efflux Carrier Gene Family in Watermelon ( Citrullus lanatus )," Agriculture, MDPI, vol. 11(5), pages 1-11, May.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1941. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.