IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v3y2012i1d10.1038_ncomms1826.html
   My bibliography  Save this article

Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster

Author

Listed:
  • Michael T. Bocker

    (DKFZ-ZMBH Alliance, German Cancer Research Center)

  • Francesca Tuorto

    (DKFZ-ZMBH Alliance, German Cancer Research Center
    Institute of Genetics and Biophysics ABT, CNR)

  • Günter Raddatz

    (DKFZ-ZMBH Alliance, German Cancer Research Center)

  • Tanja Musch

    (DKFZ-ZMBH Alliance, German Cancer Research Center)

  • Feng-Chun Yang

    (Herman B Wells Center for Pediatric Research, Indiana University Melvin and Bren Simon Cancer Center, Indiana University School of Medicine)

  • Mingjiang Xu

    (Herman B Wells Center for Pediatric Research, Indiana University Melvin and Bren Simon Cancer Center, Indiana University School of Medicine)

  • Frank Lyko

    (DKFZ-ZMBH Alliance, German Cancer Research Center)

  • Achim Breiling

    (DKFZ-ZMBH Alliance, German Cancer Research Center)

Abstract

Differentiation is accompanied by extensive epigenomic reprogramming, leading to the repression of stemness factors and the transcriptional maintenance of activated lineage-specific genes. Here we use the mammalian Hoxa cluster of developmental genes as a model system to follow changes in DNA modification patterns during retinoic acid-induced differentiation. We find the inactive cluster to be marked by defined patterns of 5-methylcytosine (5mC). Upon the induction of differentiation, the active anterior part of the cluster becomes increasingly enriched in 5-hydroxymethylcytosine (5hmC), following closely the colinear activation pattern of the gene array, which is paralleled by the reduction of 5mC. Depletion of the 5hmC generating dioxygenase Tet2 impairs the maintenance of Hoxa activity and partially restores 5mC levels. Our results indicate that gene-specific 5mC–5hmC conversion by Tet2 is crucial for the maintenance of active chromatin states at lineage-specific loci.

Suggested Citation

  • Michael T. Bocker & Francesca Tuorto & Günter Raddatz & Tanja Musch & Feng-Chun Yang & Mingjiang Xu & Frank Lyko & Achim Breiling, 2012. "Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster," Nature Communications, Nature, vol. 3(1), pages 1-12, January.
    • Handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1826
    DOI: 10.1038/ncomms1826
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms1826
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms1826?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Laure Talarmain & Matthew A. Clarke & David Shorthouse & Lilia Cabrera-Cosme & David G. Kent & Jasmin Fisher & Benjamin A. Hall, 2022. "HOXA9 has the hallmarks of a biological switch with implications in blood cancers," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:3:y:2012:i:1:d:10.1038_ncomms1826. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.