IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-58664-y.html
   My bibliography  Save this article

A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors

Author

Listed:
  • Estelle Leroy

    (Institute for Integrative Biology of the Cell (I2BC))

  • Drice Challal

    (Institute for Integrative Biology of the Cell (I2BC)
    Institut de Biologie Physico-Chimique)

  • Stéphane Pelletier

    (Institute for Integrative Biology of the Cell (I2BC))

  • Coralie Goncalves

    (Institut Jacques Monod)

  • Alexandra Menant

    (Institute for Integrative Biology of the Cell (I2BC))

  • Virginie Marchand

    (Epitranscriptomics and RNA sequencing (EpiRNA-Seq) Core Facility (SMP IBSLor) and UMR7365 IMoPA CNRS)

  • Yan Jaszczyszyn

    (Institute for Integrative Biology of the Cell (I2BC))

  • Erwin Dijk

    (Institute for Integrative Biology of the Cell (I2BC))

  • Delphine Naquin

    (Institute for Integrative Biology of the Cell (I2BC))

  • Jessica Andreani

    (Institute for Integrative Biology of the Cell (I2BC))

  • Yuri Motorin

    (Epitranscriptomics and RNA sequencing (EpiRNA-Seq) Core Facility (SMP IBSLor) and UMR7365 IMoPA CNRS)

  • Benoit Palancade

    (Institut Jacques Monod)

  • Mathieu Rougemaille

    (Institute for Integrative Biology of the Cell (I2BC))

Abstract

Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in the fission yeast long non-coding RNA mamRNA and carrying two independent cellular functions. On the one hand, snR107 guides site-specific 25S rRNA 2’-O-methylation and promotes pre-rRNA processing and 60S subunit biogenesis. On the other hand, snR107 associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct cis-motifs within snR107, including a conserved 2’-O-methylation guiding sequence. Together, our results position snR107 as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions.

Suggested Citation

  • Estelle Leroy & Drice Challal & Stéphane Pelletier & Coralie Goncalves & Alexandra Menant & Virginie Marchand & Yan Jaszczyszyn & Erwin Dijk & Delphine Naquin & Jessica Andreani & Yuri Motorin & Benoi, 2025. "A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58664-y
    DOI: 10.1038/s41467-025-58664-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-58664-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-58664-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58664-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.