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High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells

Author

Listed:
  • Camilla Rega

    (The Institute of Cancer Research)

  • Ifigenia Tsitsa

    (The Institute of Cancer Research)

  • Theodoros I. Roumeliotis

    (The Institute of Cancer Research)

  • Izabella Krystkowiak

    (The Institute of Cancer Research)

  • Maria Portillo

    (The Institute of Cancer Research)

  • Lu Yu

    (The Institute of Cancer Research)

  • Julia Vorhauser

    (The Institute of Cancer Research)

  • Jonathon Pines

    (The Institute of Cancer Research)

  • Jörg Mansfeld

    (The Institute of Cancer Research)

  • Jyoti Choudhary

    (The Institute of Cancer Research)

  • Norman E. Davey

    (The Institute of Cancer Research)

Abstract

The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in hTERT-RPE-1 cells using deep quantitative mass spectrometry by isobaric labelling. By analysing non-transformed cells and improving the temporal resolution and coverage of key cell cycle regulators, we present a dataset of cell cycle-dependent protein and phosphorylation site oscillation that offers a foundational reference for investigating cell cycle regulation. These data reveal regulatory intricacies including proteins and phosphorylation sites exhibiting cell cycle-dependent oscillation, and proteins targeted for degradation during mitotic exit. Integrated with complementary resources, our data link cycle-dependent abundance dynamics to functional changes and are accessible through the Cell Cycle database (CCdb), an interactive web-based resource for the cell cycle community.

Suggested Citation

  • Camilla Rega & Ifigenia Tsitsa & Theodoros I. Roumeliotis & Izabella Krystkowiak & Maria Portillo & Lu Yu & Julia Vorhauser & Jonathon Pines & Jörg Mansfeld & Jyoti Choudhary & Norman E. Davey, 2025. "High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57537-8
    DOI: 10.1038/s41467-025-57537-8
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