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Hidden route of protein damage through oxygen-confined photooxidation

Author

Listed:
  • Seoyoon Kim

    (Ulsan National Institute of Science and Technology)

  • Eojin Kim

    (Ulsan National Institute of Science and Technology)

  • Mingyu Park

    (Ulsan National Institute of Science and Technology)

  • Seong Ho Kim

    (Ulsan National Institute of Science and Technology)

  • Byung-Gyu Kim

    (Institute for Basic Science)

  • Seungjin Na

    (Korea Basic Science Institute)

  • Victor W. Sadongo

    (Ulsan National Institute of Science and Technology)

  • W. C. Bhashini Wijesinghe

    (Ulsan National Institute of Science and Technology)

  • Yu-Gon Eom

    (Pusan National University)

  • Gwangsu Yoon

    (Ulsan National Institute of Science and Technology)

  • Hannah Jeong

    (Ulsan National Institute of Science and Technology)

  • Eunhye Hwang

    (Ulsan National Institute of Science and Technology)

  • Chaiheon Lee

    (Ulsan National Institute of Science and Technology)

  • Kyungjae Myung

    (Institute for Basic Science
    Ulsan National Institute of Science and Technology)

  • Chae Un Kim

    (Ulsan National Institute of Science and Technology)

  • Jeong-Mo Choi

    (Pusan National University
    Pusan National University)

  • Seung Kyu Min

    (Ulsan National Institute of Science and Technology)

  • Tae-Hyuk Kwon

    (Ulsan National Institute of Science and Technology
    Ulsan National Institute of Science and Technology)

  • Duyoung Min

    (Ulsan National Institute of Science and Technology
    Ulsan National Institute of Science and Technology)

Abstract

Oxidative modifications can disrupt protein folds and functions, and are strongly associated with human aging and diseases. Conventional oxidation pathways typically involve the free diffusion of reactive oxygen species (ROS), which primarily attack the protein surface. Yet, it remains unclear whether and how internal protein folds capable of trapping oxygen (O2) contribute to oxidative damage. Here, we report a hidden pathway of protein damage, which we refer to as O2-confined photooxidation. In this process, O2 is captured in protein cavities and subsequently converted into multiple ROS, primarily mediated by tryptophan residues under blue light irradiation. The generated ROS then attack the protein interior through constrained diffusion, causing protein damage. The effects of this photooxidative reaction appear to be extensive, impacting a wide range of cellular proteins, as supported by whole-cell proteomic analysis. This photooxidative mechanism may represent a latent oxidation pathway in human tissues directly exposed to visible light, such as skin and eyes.

Suggested Citation

  • Seoyoon Kim & Eojin Kim & Mingyu Park & Seong Ho Kim & Byung-Gyu Kim & Seungjin Na & Victor W. Sadongo & W. C. Bhashini Wijesinghe & Yu-Gon Eom & Gwangsu Yoon & Hannah Jeong & Eunhye Hwang & Chaiheon , 2024. "Hidden route of protein damage through oxygen-confined photooxidation," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-55168-z
    DOI: 10.1038/s41467-024-55168-z
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    References listed on IDEAS

    as
    1. Alireza Mashaghi & Sergey Bezrukavnikov & David P. Minde & Anne S. Wentink & Roman Kityk & Beate Zachmann-Brand & Matthias P. Mayer & Günter Kramer & Bernd Bukau & Sander J. Tans, 2016. "Alternative modes of client binding enable functional plasticity of Hsp70," Nature, Nature, vol. 539(7629), pages 448-451, November.
    2. Chaiheon Lee & Mingyu Park & W. C. Bhashini Wijesinghe & Seungjin Na & Chae Gyu Lee & Eunhye Hwang & Gwangsu Yoon & Jeong Kyeong Lee & Deok-Ho Roh & Yoon Hee Kwon & Jihyeon Yang & Sebastian A. Hughes , 2024. "Oxidative photocatalysis on membranes triggers non-canonical pyroptosis," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    3. Themis Thoudam & Dipanjan Chanda & Jung Yi Lee & Min-Kyo Jung & Ibotombi Singh Sinam & Byung-Gyu Kim & Bo-Yoon Park & Woong Hee Kwon & Hyo-Jeong Kim & Myeongjin Kim & Chae Won Lim & Hoyul Lee & Yang H, 2023. "Enhanced Ca2+-channeling complex formation at the ER-mitochondria interface underlies the pathogenesis of alcohol-associated liver disease," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    4. Han Jin & Cheng Zhang & Martin Zwahlen & Kalle Feilitzen & Max Karlsson & Mengnan Shi & Meng Yuan & Xiya Song & Xiangyu Li & Hong Yang & Hasan Turkez & Linn Fagerberg & Mathias Uhlén & Adil Mardinoglu, 2023. "Systematic transcriptional analysis of human cell lines for gene expression landscape and tumor representation," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    5. Jin Kyun Kim & Cheol Lee & Seon Woo Lim & Aniruddha Adhikari & Jacob T. Andring & Robert McKenna & Cheol-Min Ghim & Chae Un Kim, 2020. "Elucidating the role of metal ions in carbonic anhydrase catalysis," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
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