IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-53738-9.html
   My bibliography  Save this article

Mammalian cell-based production of glycans, glycopeptides and glycomodules

Author

Listed:
  • Thapakorn Jaroentomeechai

    (University of Copenhagen)

  • Richard Karlsson

    (University of Copenhagen)

  • Felix Goerdeler

    (University of Copenhagen)

  • Fallen Kai Yik Teoh

    (University of Copenhagen)

  • Magnus Nørregaard Grønset

    (University of Copenhagen)

  • Dylan Wit

    (University of Copenhagen)

  • Yen-Hsi Chen

    (GlycoDisplay ApS)

  • Sanae Furukawa

    (University of Copenhagen)

  • Venetia Psomiadou

    (Radboud University Nijmegen)

  • Ramon Hurtado-Guerrero

    (University of Copenhagen
    University of Zaragoza
    Fundación ARAID)

  • Elena Ethel Vidal-Calvo

    (University of Copenhagen, Copenhagen University Hospital
    VAR2 Pharmaceuticals ApS)

  • Ali Salanti

    (University of Copenhagen, Copenhagen University Hospital)

  • Thomas J. Boltje

    (Radboud University Nijmegen)

  • Leendert J. Bos

    (EnzyTag BV)

  • Christian Wunder

    (PSL Research University, U1143 INSERM, UMR3666 CNRS)

  • Ludger Johannes

    (PSL Research University, U1143 INSERM, UMR3666 CNRS)

  • Katrine T. Schjoldager

    (University of Copenhagen)

  • Hiren J. Joshi

    (University of Copenhagen)

  • Rebecca L. Miller

    (University of Copenhagen)

  • Henrik Clausen

    (University of Copenhagen)

  • Sergey Y. Vakhrushev

    (University of Copenhagen)

  • Yoshiki Narimatsu

    (University of Copenhagen
    GlycoDisplay ApS)

Abstract

Access to defined glycans and glycoconjugates is pivotal for discovery, dissection, and harnessing of a range of biological functions orchestrated by cellular glycosylation processes and the glycome. We previously employed genetic glycoengineering by nuclease-based gene editing to develop sustainable production of designer glycoprotein therapeutics and cell-based glycan arrays that display glycans in their natural context at the cell surface. However, access to human glycans in formats and quantities that allow structural studies of molecular interactions and use of glycans in biomedical applications currently rely on chemical and chemoenzymatic syntheses associated with considerable labor, waste, and costs. Here, we develop a sustainable and scalable method for production of glycans in glycoengineered mammalian cells by employing secreted Glycocarriers with repeat glycosylation acceptor sequence motifs for different glycans. The Glycocarrier technology provides a flexible production platform for glycans in different formats, including oligosaccharides, glycopeptides, and multimeric glycomodules, and offers wide opportunities for use in bioassays and biomedical applications.

Suggested Citation

  • Thapakorn Jaroentomeechai & Richard Karlsson & Felix Goerdeler & Fallen Kai Yik Teoh & Magnus Nørregaard Grønset & Dylan Wit & Yen-Hsi Chen & Sanae Furukawa & Venetia Psomiadou & Ramon Hurtado-Guerrer, 2024. "Mammalian cell-based production of glycans, glycopeptides and glycomodules," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53738-9
    DOI: 10.1038/s41467-024-53738-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-53738-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-53738-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Hui Yang & Jinyuan Wu & Xiaochang Huang & Yunyan Zhou & Yifeng Zhang & Min Liu & Qin Liu & Shanlin Ke & Maozhang He & Hao Fu & Shaoming Fang & Xinwei Xiong & Hui Jiang & Zhe Chen & Zhongzi Wu & Huanfa, 2022. "ABO genotype alters the gut microbiota by regulating GalNAc levels in pigs," Nature, Nature, vol. 606(7913), pages 358-367, June.
    2. Rebecca Nason & Christian Büll & Andriana Konstantinidi & Lingbo Sun & Zilu Ye & Adnan Halim & Wenjuan Du & Daniel M. Sørensen & Fabien Durbesson & Sanae Furukawa & Ulla Mandel & Hiren J. Joshi & Leo , 2021. "Display of the human mucinome with defined O-glycans by gene engineered cells," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    3. Daria V. Zhernakova & Daoming Wang & Lei Liu & Sergio Andreu-Sánchez & Yue Zhang & Angel J. Ruiz-Moreno & Haoran Peng & Niels Plomp & Ángela Castillo-Izquierdo & Ranko Gacesa & Esteban A. Lopera-Maya , 2024. "Host genetic regulation of human gut microbial structural variation," Nature, Nature, vol. 625(7996), pages 813-821, January.
    4. Elena Ethel Vidal-Calvo & Anne Martin-Salazar & Swati Choudhary & Robert Dagil & Sai Sundar Rajan Raghavan & Lara Duvnjak & Mie Anemone Nordmaj & Thomas Mandel Clausen & Ann Skafte & Jan Oberkofler & , 2024. "Tumor-agnostic cancer therapy using antibodies targeting oncofetal chondroitin sulfate," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    5. Weihua Tian & Zilu Ye & Shengjun Wang & Morten Alder Schulz & Julie Coillie & Lingbo Sun & Yen-Hsi Chen & Yoshiki Narimatsu & Lars Hansen & Claus Kristensen & Ulla Mandel & Eric Paul Bennett & Siamak , 2019. "The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    6. Tomislav Čaval & Weihua Tian & Zhang Yang & Henrik Clausen & Albert J. R. Heck, 2018. "Direct quality control of glycoengineered erythropoietin variants," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    7. Yi-Ping Chiu & Yung-Chen Sun & De-Chen Qiu & Yu-Hao Lin & Yin-Quan Chen & Jean-Cheng Kuo & Jie-rong Huang, 2020. "Liquid-liquid phase separation and extracellular multivalent interactions in the tale of galectin-3," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Joann Chongsaritsinsuk & Alexandra D. Steigmeyer & Keira E. Mahoney & Mia A. Rosenfeld & Taryn M. Lucas & Courtney M. Smith & Alice Li & Deniz Ince & Fiona L. Kearns & Alexandria S. Battison & Marie A, 2023. "Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Xinkai Tong & Dong Chen & Jianchao Hu & Shiyao Lin & Ziqi Ling & Huashui Ai & Zhiyan Zhang & Lusheng Huang, 2023. "Accurate haplotype construction and detection of selection signatures enabled by high quality pig genome sequences," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Janneke Elzinga & Yoshiki Narimatsu & Noortje Haan & Henrik Clausen & Willem M. Vos & Hanne L. P. Tytgat, 2024. "Binding of Akkermansia muciniphila to mucin is O-glycan specific," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    4. Daniel Madriz Sørensen & Christian Büll & Thomas D. Madsen & Erandi Lira-Navarrete & Thomas Mandel Clausen & Alex E. Clark & Aaron F. Garretson & Richard Karlsson & Johan F. A. Pijnenborg & Xin Yin & , 2023. "Identification of global inhibitors of cellular glycosylation," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    5. Luis F. Schachner & Christopher Mullen & Wilson Phung & Joshua D. Hinkle & Michelle Irwin Beardsley & Tracy Bentley & Peter Day & Christina Tsai & Siddharth Sukumaran & Tomasz Baginski & Danielle DiCa, 2024. "Exposing the molecular heterogeneity of glycosylated biotherapeutics," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    6. Yunyan Zhou & Jingquan Li & Fei Huang & Huashui Ai & Jun Gao & Congying Chen & Lusheng Huang, 2023. "Characterization of the pig lower respiratory tract antibiotic resistome," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53738-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.