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Efficient conversion of hemicellulose into high-value product and electric power by enzyme-engineered bacterial consortia

Author

Listed:
  • Bo Liang

    (Qingdao Agricultural University)

  • Jing Yang

    (Qingdao Agricultural University)

  • Chen-Fei Meng

    (Qingdao Agricultural University)

  • Ya-Ru Zhang

    (Qingdao University)

  • Lu Wang

    (Qingdao Agricultural University)

  • Li Zhang

    (Qingdao Agricultural University)

  • Jia Liu

    (Qingdao Agricultural University)

  • Zhen-Chao Li

    (Qingdao University)

  • Serge Cosnier

    (44-100
    44-100
    DCM UMR 5250)

  • Ai-Hua Liu

    (Qingdao University)

  • Jian-Ming Yang

    (Qingdao Agricultural University)

Abstract

As an abundant agricultural and forestry biomass resource, hemicelluloses are hard to be effectively degraded and utilized by microorganisms due to the constraints of membrane and metabolic regulations. Herein, we report a synthetic extracellular metabolic pathway with hemicellulose-degrading-enzymes controllably displayed on Escherichia coli surface as engineered bacterial consortia members for efficient utilization of xylan, the most abundant component in hemicellulose. Further, we develop a hemicellulose/O2 microbial fuel cell (MFC) configuring of enzyme-engineered bacterial consortia based bioanode and bacterial-displayed laccase based biocathode. The optimized MFC exhibited an open-circuit voltage of 0.71 V and a maximum power density (Pmax) of 174.33 ± 4.56 µW cm−2. Meanwhile, 46.6% (w/w) α-ketoglutarate was produced in this hemicellulose fed-MFC. Besides, the MFC retained over 95% of the Pmax during 6 days’ operation. Therefore, this work establishes an effective and sustainable one-pot process for catalyzing renewable biomass into high-value products and electricity in an environmentally-friendly way.

Suggested Citation

  • Bo Liang & Jing Yang & Chen-Fei Meng & Ya-Ru Zhang & Lu Wang & Li Zhang & Jia Liu & Zhen-Chao Li & Serge Cosnier & Ai-Hua Liu & Jian-Ming Yang, 2024. "Efficient conversion of hemicellulose into high-value product and electric power by enzyme-engineered bacterial consortia," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53129-0
    DOI: 10.1038/s41467-024-53129-0
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    References listed on IDEAS

    as
    1. Zhiguang Zhu & Tsz Kin Tam & Fangfang Sun & Chun You & Y. -H. Percival Zhang, 2014. "A high-energy-density sugar biobattery based on a synthetic enzymatic pathway," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
    2. Lisa A. Schulte & Bruce E. Dale & Stefano Bozzetto & Matt Liebman & Glaucia M. Souza & Nick Haddad & Tom L. Richard & Bruno Basso & Robert C. Brown & Jorge A. Hilbert & J. Gordon Arbuckle, 2022. "Meeting global challenges with regenerative agriculture producing food and energy," Nature Sustainability, Nature, vol. 5(5), pages 384-388, May.
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