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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

Author

Listed:
  • Olga Iwańska

    (Pawinskiego 5a)

  • Przemysław Latoch

    (Pawinskiego 5a)

  • Natalia Kopik

    (Marii Curie-Skłodowskiej 5)

  • Mariia Kovalenko

    (Pawinskiego 5a)

  • Małgorzata Lichocka

    (Pawinskiego 5a)

  • Remigiusz Serwa

    (M. Flisa 6)

  • Agata L. Starosta

    (Pawinskiego 5a)

Abstract

The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation.

Suggested Citation

  • Olga Iwańska & Przemysław Latoch & Natalia Kopik & Mariia Kovalenko & Małgorzata Lichocka & Remigiusz Serwa & Agata L. Starosta, 2024. "Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51654-6
    DOI: 10.1038/s41467-024-51654-6
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    References listed on IDEAS

    as
    1. Jung-Ho Shin & John D. Helmann, 2016. "Molecular logic of the Zur-regulated zinc deprivation response in Bacillus subtilis," Nature Communications, Nature, vol. 7(1), pages 1-9, November.
    2. Kevin Rychel & Anand V. Sastry & Bernhard O. Palsson, 2020. "Machine learning uncovers independently regulated modules in the Bacillus subtilis transcriptome," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    3. Grace E. Johnson & Jean-Benoît Lalanne & Michelle L. Peters & Gene-Wei Li, 2020. "Functionally uncoupled transcription–translation in Bacillus subtilis," Nature, Nature, vol. 585(7823), pages 124-128, September.
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