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Chromosome-scale pearl millet genomes reveal CLAMT1b as key determinant of strigolactone pattern and Striga susceptibility

Author

Listed:
  • Hendrik N. J. Kuijer

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Jian You Wang

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Salim Bougouffa

    (King Abdullah University of Science and Technology (KAUST))

  • Michael Abrouk

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Muhammad Jamil

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Roberto Incitti

    (King Abdullah University of Science and Technology (KAUST))

  • Intikhab Alam

    (King Abdullah University of Science and Technology (KAUST))

  • Aparna Balakrishna

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Derry Alvarez

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Cristina Votta

    (University of Torino; Viale Mattioli 25)

  • Guan-Ting Erica Chen

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Claudio Martínez

    (Facultade de Química and CINBIO)

  • Andrea Zuccolo

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST)
    Sant’Anna School of Advanced Studies)

  • Lamis Berqdar

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Salim Sioud

    (King Abdullah University of Science and Technology (KAUST))

  • Valentina Fiorilli

    (University of Torino; Viale Mattioli 25)

  • Angel R. Lera

    (Facultade de Química and CINBIO)

  • Luisa Lanfranco

    (University of Torino; Viale Mattioli 25)

  • Takashi Gojobori

    (King Abdullah University of Science and Technology (KAUST))

  • Rod A. Wing

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Simon G. Krattinger

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

  • Xin Gao

    (King Abdullah University of Science and Technology (KAUST))

  • Salim Al-Babili

    (King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST)
    King Abdullah University of Science and Technology (KAUST))

Abstract

The yield of pearl millet, a resilient cereal crop crucial for African food security, is severely impacted by the root parasitic weed Striga hermonthica, which requires host-released hormones, called strigolactones (SLs), for seed germination. Herein, we identify four SLs present in the Striga-susceptible line SOSAT-C88-P10 (P10) but absent in the resistant 29Aw (Aw). We generate chromosome-scale genome assemblies, including four gapless chromosomes for each line. The Striga-resistant Aw lacks a 0.7 Mb genome segment containing two putative CARLACTONOIC ACID METHYLTRANSFERASE1 (CLAMT1) genes, which may contribute to SL biosynthesis. Functional assays show that P10CLAMT1b produces the SL-biosynthesis intermediate methyl carlactonoate (MeCLA) and that MeCLA is the precursor of P10-specific SLs. Screening a diverse pearl millet panel confirms the pivotal role of the CLAMT1 section for SL diversity and Striga susceptibility. Our results reveal a reason for Striga susceptibility in pearl millet and pave the way for generating resistant lines through marker-assisted breeding or direct genetic modification.

Suggested Citation

  • Hendrik N. J. Kuijer & Jian You Wang & Salim Bougouffa & Michael Abrouk & Muhammad Jamil & Roberto Incitti & Intikhab Alam & Aparna Balakrishna & Derry Alvarez & Cristina Votta & Guan-Ting Erica Chen , 2024. "Chromosome-scale pearl millet genomes reveal CLAMT1b as key determinant of strigolactone pattern and Striga susceptibility," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51189-w
    DOI: 10.1038/s41467-024-51189-w
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    References listed on IDEAS

    as
    1. Jian You Wang & Imran Haider & Muhammad Jamil & Valentina Fiorilli & Yoshimoto Saito & Jianing Mi & Lina Baz & Boubacar A. Kountche & Kun-Peng Jia & Xiujie Guo & Aparna Balakrishna & Valentine O. Ntui, 2019. "The apocarotenoid metabolite zaxinone regulates growth and strigolactone biosynthesis in rice," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    2. Jinying Cui & Noriko Nishide & Kiyoshi Mashiguchi & Kana Kuroha & Masayuki Miya & Kazuhiko Sugimoto & Jun-Ichi Itoh & Shinjiro Yamaguchi & Takeshi Izawa, 2023. "Fertilization controls tiller numbers via transcriptional regulation of a MAX1-like gene in rice cultivation," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
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