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Motion blur microscopy: in vitro imaging of cell adhesion dynamics in whole blood flow

Author

Listed:
  • Utku Goreke

    (Case Western Reserve University)

  • Ayesha Gonzales

    (Case Western Reserve University)

  • Brandon Shipley

    (Case Western Reserve University)

  • Madeleine Tincher

    (Case Western Reserve University)

  • Oshin Sharma

    (Case Western Reserve University)

  • William J. Wulftange

    (Case Western Reserve University)

  • Yuncheng Man

    (Case Western Reserve University)

  • Ran An

    (Case Western Reserve University)

  • Michael Hinczewski

    (Case Western Reserve University)

  • Umut A. Gurkan

    (Case Western Reserve University
    Case Western Reserve University)

Abstract

Imaging and characterizing the dynamics of cellular adhesion in blood samples is of fundamental importance in understanding biological function. In vitro microscopy methods are widely used for this task but typically require diluting the blood with a buffer to allow for transmission of light. However, whole blood provides crucial signaling cues that influence adhesion dynamics, which means that conventional approaches lack the full physiological complexity of living microvasculature. We can reliably image cell interactions in microfluidic channels during whole blood flow by motion blur microscopy (MBM) in vitro and automate image analysis using machine learning. MBM provides a low cost, easy to implement alternative to intravital microscopy, for rapid data generation where understanding cell interactions, adhesion, and motility is crucial. MBM is generalizable to studies of various diseases, including cancer, blood disorders, thrombosis, inflammatory and autoimmune diseases, as well as providing rich datasets for theoretical modeling of adhesion dynamics.

Suggested Citation

  • Utku Goreke & Ayesha Gonzales & Brandon Shipley & Madeleine Tincher & Oshin Sharma & William J. Wulftange & Yuncheng Man & Ran An & Michael Hinczewski & Umut A. Gurkan, 2024. "Motion blur microscopy: in vitro imaging of cell adhesion dynamics in whole blood flow," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51014-4
    DOI: 10.1038/s41467-024-51014-4
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    References listed on IDEAS

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    1. Jinsung Yang & Simon J. L. Petitjean & Melanie Koehler & Qingrong Zhang & Andra C. Dumitru & Wenzhang Chen & Sylvie Derclaye & Stéphane P. Vincent & Patrice Soumillion & David Alsteens, 2020. "Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
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