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CryoET reveals actin filaments within platelet microtubules

Author

Listed:
  • Chisato Tsuji

    (University of Bristol)

  • Marston Bradshaw

    (University of Bristol)

  • Megan F. Allen

    (University of Bristol)

  • Molly L. Jackson

    (University of Bristol)

  • Judith Mantell

    (University of Bristol)

  • Ufuk Borucu

    (University of Bristol)

  • Alastair W. Poole

    (University of Bristol)

  • Paul Verkade

    (University of Bristol)

  • Ingeborg Hers

    (University of Bristol)

  • Danielle M. Paul

    (University of Bristol)

  • Mark P. Dodding

    (University of Bristol)

Abstract

Crosstalk between the actin and microtubule cytoskeletons is important for many cellular processes. Recent studies have shown that microtubules and F-actin can assemble to form a composite structure where F-actin occupies the microtubule lumen. Whether these cytoskeletal hybrids exist in physiological settings and how they are formed is unclear. Here, we show that the short-crossover Class I actin filament previously identified inside microtubules in human HAP1 cells is cofilin-bound F-actin. Lumenal F-actin can be reconstituted in vitro, but cofilin is not essential. Moreover, actin filaments with both cofilin-bound and canonical morphologies reside within human platelet microtubules under physiological conditions. We propose that stress placed upon the microtubule network during motor-driven microtubule looping and sliding may facilitate the incorporation of actin into microtubules.

Suggested Citation

  • Chisato Tsuji & Marston Bradshaw & Megan F. Allen & Molly L. Jackson & Judith Mantell & Ufuk Borucu & Alastair W. Poole & Paul Verkade & Ingeborg Hers & Danielle M. Paul & Mark P. Dodding, 2024. "CryoET reveals actin filaments within platelet microtubules," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50424-8
    DOI: 10.1038/s41467-024-50424-8
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    References listed on IDEAS

    as
    1. Josie L. Ferreira & Vojtěch Pražák & Daven Vasishtan & Marc Siggel & Franziska Hentzschel & Annika M. Binder & Emma Pietsch & Jan Kosinski & Friedrich Frischknecht & Tim W. Gilberger & Kay Grünewald, 2023. "Variable microtubule architecture in the malaria parasite," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Matthew J. Reynolds & Carla Hachicho & Ayala G. Carl & Rui Gong & Gregory M. Alushin, 2022. "Bending forces and nucleotide state jointly regulate F-actin structure," Nature, Nature, vol. 611(7935), pages 380-386, November.
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