IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-50045-1.html
   My bibliography  Save this article

Self-powered triboelectric-responsive microneedles with controllable release of optogenetically engineered extracellular vesicles for intervertebral disc degeneration repair

Author

Listed:
  • Weifeng Zhang

    (Huazhong University of Science and Technology)

  • Xuan Qin

    (Soochow University)

  • Gaocai Li

    (Huazhong University of Science and Technology)

  • Xingyu Zhou

    (Huazhong University of Science and Technology)

  • Hongyang Li

    (Soochow University)

  • Di Wu

    (Huazhong University of Science and Technology)

  • Yu Song

    (Huazhong University of Science and Technology)

  • Kangcheng Zhao

    (Huazhong University of Science and Technology)

  • Kun Wang

    (Huazhong University of Science and Technology)

  • Xiaobo Feng

    (Huazhong University of Science and Technology)

  • Lei Tan

    (Huazhong University of Science and Technology)

  • Bingjin Wang

    (Huazhong University of Science and Technology)

  • Xuhui Sun

    (Soochow University)

  • Zhen Wen

    (Soochow University)

  • Cao Yang

    (Huazhong University of Science and Technology)

Abstract

Excessive exercise is an etiological factor of intervertebral disc degeneration (IVDD). Engineered extracellular vesicles (EVs) exhibit excellent therapeutic potential for disease-modifying treatments. Herein, we fabricate an exercise self-powered triboelectric-responsive microneedle (MN) assay with the sustainable release of optogenetically engineered EVs for IVDD repair. Mechanically, exercise promotes cytosolic DNA sensing-mediated inflammatory activation in senescent nucleus pulposus (NP) cells (the master cell population for IVD homeostasis maintenance), which accelerates IVDD. TREX1 serves as a crucial nuclease, and disassembly of TRAM1-TREX1 complex disrupts the subcellular localization of TREX1, triggering TREX1-dependent genomic DNA damage during NP cell senescence. Optogenetically engineered EVs deliver TRAM1 protein into senescent NP cells, which effectively reconstructs the elimination function of TREX1. Triboelectric nanogenerator (TENG) harvests mechanical energy and triggers the controllable release of engineered EVs. Notably, an optogenetically engineered EV-based targeting treatment strategy is used for the treatment of IVDD, showing promising clinical potential for the treatment of degeneration-associated disorders.

Suggested Citation

  • Weifeng Zhang & Xuan Qin & Gaocai Li & Xingyu Zhou & Hongyang Li & Di Wu & Yu Song & Kangcheng Zhao & Kun Wang & Xiaobo Feng & Lei Tan & Bingjin Wang & Xuhui Sun & Zhen Wen & Cao Yang, 2024. "Self-powered triboelectric-responsive microneedles with controllable release of optogenetically engineered extracellular vesicles for intervertebral disc degeneration repair," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50045-1
    DOI: 10.1038/s41467-024-50045-1
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-50045-1
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-50045-1?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Akiko Takahashi & Tze Mun Loo & Ryo Okada & Fumitaka Kamachi & Yoshihiro Watanabe & Masahiro Wakita & Sugiko Watanabe & Shimpei Kawamoto & Kenichi Miyata & Glen N. Barber & Naoko Ohtani & Eiji Hara, 2018. "Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    2. Nambin Yim & Seung-Wook Ryu & Kyungsun Choi & Kwang Ryeol Lee & Seunghee Lee & Hojun Choi & Jeongjin Kim & Mohammed R. Shaker & Woong Sun & Ji-Ho Park & Daesoo Kim & Won Do Heo & Chulhee Choi, 2016. "Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module," Nature Communications, Nature, vol. 7(1), pages 1-9, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Toshiyuki Ko & Seitaro Nomura & Shintaro Yamada & Kanna Fujita & Takanori Fujita & Masahiro Satoh & Chio Oka & Manami Katoh & Masamichi Ito & Mikako Katagiri & Tatsuro Sassa & Bo Zhang & Satoshi Hatsu, 2022. "Cardiac fibroblasts regulate the development of heart failure via Htra3-TGF-β-IGFBP7 axis," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Nanase Igarashi & Kenichi Miyata & Tze Mun Loo & Masatomo Chiba & Aki Hanyu & Mika Nishio & Hiroko Kawasaki & Hao Zheng & Shinya Toyokuni & Shunsuke Kon & Keiji Moriyama & Yasuyuki Fujita & Akiko Taka, 2022. "Hepatocyte growth factor derived from senescent cells attenuates cell competition-induced apical elimination of oncogenic cells," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    3. Vanessa López-Polo & Mate Maus & Emmanouil Zacharioudakis & Miguel Lafarga & Camille Stephan-Otto Attolini & Francisco D. M. Marques & Marta Kovatcheva & Evripidis Gavathiotis & Manuel Serrano, 2024. "Release of mitochondrial dsRNA into the cytosol is a key driver of the inflammatory phenotype of senescent cells," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    4. Wenyi Zheng & Julia Rädler & Helena Sork & Zheyu Niu & Samantha Roudi & Jeremy P. Bost & André Görgens & Ying Zhao & Doste R. Mamand & Xiuming Liang & Oscar P. B. Wiklander & Taavi Lehto & Dhanu Gupta, 2023. "Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50045-1. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.