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Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging

Author

Listed:
  • Le Wang

    (Boston University)

  • Haonan Lin

    (Boston University)

  • Yifan Zhu

    (Boston University)

  • Xiaowei Ge

    (Boston University)

  • Mingsheng Li

    (Boston University)

  • Jianing Liu

    (Boston University)

  • Fukai Chen

    (Boston University)

  • Meng Zhang

    (Boston University)

  • Ji-Xin Cheng

    (Boston University
    Boston University
    Boston University)

Abstract

Photothermal microscopy is a highly sensitive pump-probe method for mapping nanostructures and molecules through the detection of local thermal gradients. While visible photothermal microscopy and mid-infrared photothermal microscopy techniques have been developed, they possess inherent limitations. These techniques either lack chemical specificity or encounter significant light attenuation caused by water absorption. Here, we present an overtone photothermal (OPT) microscopy technique that offers high chemical specificity, detection sensitivity, and spatial resolution by employing a visible probe for local heat detection in the C-H overtone region. We demonstrate its capability for high-fidelity chemical imaging of polymer nanostructures, depth-resolved intracellular chemical mapping of cancer cells, and imaging of multicellular C. elegans organisms and highly scattering brain tissues. By bridging the gap between visible and mid-infrared photothermal microscopy, OPT establishes a new modality for high-resolution and high-sensitivity chemical imaging. This advancement complements large-scale shortwave infrared imaging approaches, facilitating multiscale structural and chemical investigations of materials and biological metabolism.

Suggested Citation

  • Le Wang & Haonan Lin & Yifan Zhu & Xiaowei Ge & Mingsheng Li & Jianing Liu & Fukai Chen & Meng Zhang & Ji-Xin Cheng, 2024. "Overtone photothermal microscopy for high-resolution and high-sensitivity vibrational imaging," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49691-2
    DOI: 10.1038/s41467-024-49691-2
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    References listed on IDEAS

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    1. Haonan Lin & Hyeon Jeong Lee & Nathan Tague & Jean-Baptiste Lugagne & Cheng Zong & Fengyuan Deng & Jonghyeon Shin & Lei Tian & Wilson Wong & Mary J. Dunlop & Ji-Xin Cheng, 2021. "Microsecond fingerprint stimulated Raman spectroscopic imaging by ultrafast tuning and spatial-spectral learning," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Kevin Yeh & Ishaan Sharma & Kianoush Falahkheirkhah & Matthew P. Confer & Andres C. Orr & Yen-Ting Liu & Yamuna Phal & Ruo-Jing Ho & Manu Mehta & Ankita Bhargava & Wenyan Mei & Georgina Cheng & John C, 2023. "Infrared spectroscopic laser scanning confocal microscopy for whole-slide chemical imaging," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Yanyu Zhao & Anahita Pilvar & Anup Tank & Hannah Peterson & John Jiang & Jon C. Aster & John Paul Dumas & Mark C. Pierce & Darren Roblyer, 2020. "Shortwave-infrared meso-patterned imaging enables label-free mapping of tissue water and lipid content," Nature Communications, Nature, vol. 11(1), pages 1-12, December.
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