IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-49014-5.html
   My bibliography  Save this article

Light-evoked deformations in rod photoreceptors, pigment epithelium and subretinal space revealed by prolonged and multilayered optoretinography

Author

Listed:
  • Bingyao Tan

    (Singapore National Eye Centre
    SERI-NTU Advanced Ocular Engineering (STANCE) Program)

  • Huakun Li

    (Nanyang Technological University)

  • Yueming Zhuo

    (Stanford University
    Stanford University)

  • Le Han

    (Singapore National Eye Centre
    SERI-NTU Advanced Ocular Engineering (STANCE) Program)

  • Rajeshkumar Mupparapu

    (Singapore National Eye Centre
    SERI-NTU Advanced Ocular Engineering (STANCE) Program)

  • Davide Nanni

    (Nanyang Technological University)

  • Veluchamy Amutha Barathi

    (Singapore National Eye Centre
    National University of Singapore and National University Health System
    Duke-NUS Medical School)

  • Daniel Palanker

    (Stanford University
    Stanford University)

  • Leopold Schmetterer

    (Singapore National Eye Centre
    SERI-NTU Advanced Ocular Engineering (STANCE) Program
    Nanyang Technological University
    National University of Singapore and National University Health System)

  • Tong Ling

    (Singapore National Eye Centre
    SERI-NTU Advanced Ocular Engineering (STANCE) Program
    Nanyang Technological University
    Nanyang Technological University)

Abstract

Phototransduction involves changes in concentration of ions and other solutes within photoreceptors and in subretinal space, which affect osmotic pressure and the associated water flow. Corresponding expansion and contraction of cellular layers can be imaged using optoretinography (ORG), based on phase-resolved optical coherence tomography (OCT). Until now, ORG could reliably detect only photoisomerization and phototransduction in photoreceptors, primarily in cones under bright stimuli. Here, by employing a phase-restoring subpixel motion correction algorithm, which enables imaging of the nanometer-scale tissue dynamics during minute-long recordings, and unsupervised learning of spatiotemporal patterns, we discover optical signatures of the other retinal structures’ response to visual stimuli. These include inner and outer segments of rod photoreceptors, retinal pigment epithelium, and subretinal space in general. The high sensitivity of our technique enables detection of the retinal responses to dim stimuli: down to 0.01% bleach level, corresponding to natural levels of scotopic illumination. We also demonstrate that with a single flash, the optoretinogram can map retinal responses across a 12° field of view, potentially replacing multifocal electroretinography. This technique expands the diagnostic capabilities and practical applicability of optoretinography, providing an alternative to electroretinography, while combining structural and functional retinal imaging in the same OCT machine.

Suggested Citation

  • Bingyao Tan & Huakun Li & Yueming Zhuo & Le Han & Rajeshkumar Mupparapu & Davide Nanni & Veluchamy Amutha Barathi & Daniel Palanker & Leopold Schmetterer & Tong Ling, 2024. "Light-evoked deformations in rod photoreceptors, pigment epithelium and subretinal space revealed by prolonged and multilayered optoretinography," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49014-5
    DOI: 10.1038/s41467-024-49014-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-49014-5
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-024-49014-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Bing-Yi Wang & Zhijie Charles Chen & Mohajeet Bhuckory & Tiffany Huang & Andrew Shin & Valentina Zuckerman & Elton Ho & Ethan Rosenfeld & Ludwig Galambos & Theodore Kamins & Keith Mathieson & Daniel P, 2022. "Electronic photoreceptors enable prosthetic visual acuity matching the natural resolution in rats," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Franklin Leong & Babak Rahmani & Demetri Psaltis & Christophe Moser & Diego Ghezzi, 2024. "An actor-model framework for visual sensory encoding," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Gengxi Lu & Chen Gong & Yizhe Sun & Xuejun Qian & Deepthi S. Rajendran Nair & Runze Li & Yushun Zeng & Jie Ji & Junhang Zhang & Haochen Kang & Laiming Jiang & Jiawen Chen & Chi-Feng Chang & Biju B. Th, 2024. "Noninvasive imaging-guided ultrasonic neurostimulation with arbitrary 2D patterns and its application for high-quality vision restoration," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49014-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.