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Spatially resolved multiomics on the neuronal effects induced by spaceflight in mice

Author

Listed:
  • Yuvarani Masarapu

    (KTH Royal Institute of Technology)

  • Egle Cekanaviciute

    (NASA Ames Research Center)

  • Zaneta Andrusivova

    (KTH Royal Institute of Technology)

  • Jakub O. Westholm

    (Science for Life Laboratory)

  • Åsa Björklund

    (Uppsala University)

  • Robin Fallegger

    (Bioquant)

  • Pau Badia-i-Mompel

    (Bioquant
    Cellzome)

  • Valery Boyko

    (NASA Ames Research Center
    Bionetics)

  • Shubha Vasisht

    (The Children’s Hospital of Philadelphia Research Institute)

  • Amanda Saravia-Butler

    (NASA Ames Research Center)

  • Samrawit Gebre

    (NASA Ames Research Center)

  • Enikő Lázár

    (KTH Royal Institute of Technology
    Karolinska Institute)

  • Marta Graziano

    (Biomedicum)

  • Solène Frapard

    (KTH Royal Institute of Technology)

  • Robert G. Hinshaw

    (NASA Ames Research Center)

  • Olaf Bergmann

    (Karolinska Institute
    Department of Pharmacology and Toxicology University Medical Center Goettingen)

  • Deanne M. Taylor

    (The Children’s Hospital of Philadelphia Research Institute
    The University of Pennsylvania Perelman School of Medicine)

  • Douglas C. Wallace

    (The University of Pennsylvania Perelman School of Medicine)

  • Christer Sylvén

    (Karolinska Institute)

  • Konstantinos Meletis

    (Biomedicum)

  • Julio Saez-Rodriguez

    (Bioquant)

  • Jonathan M. Galazka

    (NASA Ames Research Center)

  • Sylvain V. Costes

    (NASA Ames Research Center)

  • Stefania Giacomello

    (KTH Royal Institute of Technology)

Abstract

Impairment of the central nervous system (CNS) poses a significant health risk for astronauts during long-duration space missions. In this study, we employed an innovative approach by integrating single-cell multiomics (transcriptomics and chromatin accessibility) with spatial transcriptomics to elucidate the impact of spaceflight on the mouse brain in female mice. Our comparative analysis between ground control and spaceflight-exposed animals revealed significant alterations in essential brain processes including neurogenesis, synaptogenesis and synaptic transmission, particularly affecting the cortex, hippocampus, striatum and neuroendocrine structures. Additionally, we observed astrocyte activation and signs of immune dysfunction. At the pathway level, some spaceflight-induced changes in the brain exhibit similarities with neurodegenerative disorders, marked by oxidative stress and protein misfolding. Our integrated spatial multiomics approach serves as a stepping stone towards understanding spaceflight-induced CNS impairments at the level of individual brain regions and cell types, and provides a basis for comparison in future spaceflight studies. For broader scientific impact, all datasets from this study are available through an interactive data portal, as well as the National Aeronautics and Space Administration (NASA) Open Science Data Repository (OSDR).

Suggested Citation

  • Yuvarani Masarapu & Egle Cekanaviciute & Zaneta Andrusivova & Jakub O. Westholm & Åsa Björklund & Robin Fallegger & Pau Badia-i-Mompel & Valery Boyko & Shubha Vasisht & Amanda Saravia-Butler & Samrawi, 2024. "Spatially resolved multiomics on the neuronal effects induced by spaceflight in mice," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48916-8
    DOI: 10.1038/s41467-024-48916-8
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    References listed on IDEAS

    as
    1. Zhuoxuan Li & Tianjie Wang & Pentao Liu & Yuanhua Huang, 2023. "SpatialDM for rapid identification of spatially co-expressed ligand–receptor and revealing cell–cell communication patterns," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Michael Schubert & Bertram Klinger & Martina Klünemann & Anja Sieber & Florian Uhlitz & Sascha Sauer & Mathew J. Garnett & Nils Blüthgen & Julio Saez-Rodriguez, 2018. "Perturbation-response genes reveal signaling footprints in cancer gene expression," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
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