IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-48724-0.html
   My bibliography  Save this article

HDAC activity is dispensable for repression of cell-cycle genes by DREAM and E2F:RB complexes

Author

Listed:
  • Alison K. Barrett

    (University of California)

  • Manisha R. Shingare

    (University of California)

  • Andreas Rechtsteiner

    (University of California)

  • Kelsie M. Rodriguez

    (University of California)

  • Quynh N. Le

    (University of California)

  • Tilini U. Wijeratne

    (University of California)

  • Corbin E. Mitchell

    (University of California)

  • Miles W. Membreno

    (University of California)

  • Seth M. Rubin

    (University of California)

  • Gerd A. Müller

    (University of California)

Abstract

Histone deacetylases (HDACs) play a crucial role in transcriptional regulation and are implicated in various diseases, including cancer. They are involved in histone tail deacetylation and canonically linked to transcriptional repression. Previous studies suggested that HDAC recruitment to cell-cycle gene promoters via the retinoblastoma (RB) protein or the DREAM complex through SIN3B is essential for G1/S and G2/M gene repression during cell-cycle arrest and exit. Here we investigate the interplay among DREAM, RB, SIN3 proteins, and HDACs in the context of cell-cycle gene repression. Knockout of SIN3B does not globally derepress cell-cycle genes in non-proliferating HCT116 and C2C12 cells. Loss of SIN3A/B moderately upregulates several cell-cycle genes in HCT116 cells but does so independently of DREAM/RB. HDAC inhibition does not induce general upregulation of RB/DREAM target genes in arrested transformed or non-transformed cells. Our findings suggest that E2F:RB and DREAM complexes can repress cell-cycle genes without relying on HDAC activity.

Suggested Citation

  • Alison K. Barrett & Manisha R. Shingare & Andreas Rechtsteiner & Kelsie M. Rodriguez & Quynh N. Le & Tilini U. Wijeratne & Corbin E. Mitchell & Miles W. Membreno & Seth M. Rubin & Gerd A. Müller, 2024. "HDAC activity is dispensable for repression of cell-cycle genes by DREAM and E2F:RB complexes," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48724-0
    DOI: 10.1038/s41467-024-48724-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-48724-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-48724-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. L. Magnaghi-Jaulin & R. Groisman & I. Naguibneva & P. Robin & S. Lorain & J. P. Le Villain & F. Troalen & D. Trouche & A. Harel-Bellan, 1998. "Retinoblastoma protein represses transcription by recruiting a histone deacetylase," Nature, Nature, vol. 391(6667), pages 601-605, February.
    2. Anushweta Asthana & Parameshwaran Ramanan & Alexander Hirschi & Keelan Z. Guiley & Tilini U. Wijeratne & Robert Shelansky & Michael J. Doody & Haritha Narasimhan & Hinrich Boeger & Sarvind Tripathi & , 2022. "The MuvB complex binds and stabilizes nucleosomes downstream of the transcription start site of cell-cycle dependent genes," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Alexander Brehm & Eric A. Miska & Dennis J. McCance & Juliet L. Reid & Andrew J. Bannister & Tony Kouzarides, 1998. "Retinoblastoma protein recruits histone deacetylase to repress transcription," Nature, Nature, vol. 391(6667), pages 597-601, February.
    4. Marios G. Koliopoulos & Reyhan Muhammad & Theodoros I. Roumeliotis & Fabienne Beuron & Jyoti S. Choudhary & Claudio Alfieri, 2022. "Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    5. Aimee H. Marceau & Jessica G. Felthousen & Paul D. Goetsch & Audra N. Iness & Hsiau-Wei Lee & Sarvind M. Tripathi & Susan Strome & Larisa Litovchick & Seth M. Rubin, 2016. "Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters," Nature Communications, Nature, vol. 7(1), pages 1-11, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Marios G. Koliopoulos & Reyhan Muhammad & Theodoros I. Roumeliotis & Fabienne Beuron & Jyoti S. Choudhary & Claudio Alfieri, 2022. "Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Mandy S. M. Wan & Reyhan Muhammad & Marios G. Koliopoulos & Theodoros I. Roumeliotis & Jyoti S. Choudhary & Claudio Alfieri, 2023. "Mechanism of assembly, activation and lysine selection by the SIN3B histone deacetylase complex," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48724-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.