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Mechanical-scan-free multicolor super-resolution imaging with diffractive spot array illumination

Author

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  • Ning Xu

    (Tsinghua University)

  • Sarah E. Bohndiek

    (University of Cambridge
    University of Cambridge)

  • Zexing Li

    (University of Cambridge)

  • Cilong Zhang

    (Tsinghua University)

  • Qiaofeng Tan

    (Tsinghua University)

Abstract

Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multicolor imaging methods with high-quality illumination are currently lacking. In this paper, we present for the first time Mechanical-scan-free multiColor Super-resolution Microscopy (MCoSM) with spot array illumination, which enables mechanical-scan-free super-resolution imaging with adjustable resolution and a good effective field-of-view based on spatial light modulators. Through 100–2,500 s super-resolution spot illumination with different effective fields of view for imaging, we demonstrate the adjustable capacity of MCoSM. MCoSM extends existing spectral imaging capabilities through a time-sharing process involving different color illumination with phase-shift scanning while retaining the spatial flexibility of super-resolution imaging with diffractive spot array illumination. To demonstrate the prospects of MCoSM, we perform four-color imaging of fluorescent beads at high resolution. MCoSM provides a versatile platform for studying molecular interactions in complex samples at the nanoscale level.

Suggested Citation

  • Ning Xu & Sarah E. Bohndiek & Zexing Li & Cilong Zhang & Qiaofeng Tan, 2024. "Mechanical-scan-free multicolor super-resolution imaging with diffractive spot array illumination," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48482-z
    DOI: 10.1038/s41467-024-48482-z
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    1. Yicong Wu & Xiaofei Han & Yijun Su & Melissa Glidewell & Jonathan S. Daniels & Jiamin Liu & Titas Sengupta & Ivan Rey-Suarez & Robert Fischer & Akshay Patel & Christian Combs & Junhui Sun & Xufeng Wu , 2021. "Multiview confocal super-resolution microscopy," Nature, Nature, vol. 600(7888), pages 279-284, December.
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