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An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis

Author

Listed:
  • Jiawen Wen

    (Ministry of Agriculture, BGI Research
    University of Chinese Academy of Sciences)

  • Yayu Wang

    (Ministry of Agriculture, BGI Research)

  • Xu Lu

    (China Pharmaceutical University)

  • Huimin Pan

    (South China Agricultural University)

  • Dian Jin

    (China Pharmaceutical University)

  • Jialing Wen

    (Ministry of Agriculture, BGI Research
    University of Chinese Academy of Sciences)

  • Canzhi Jin

    (Ministry of Agriculture, BGI Research
    University of Chinese Academy of Sciences)

  • Sunil Kumar Sahu

    (Ministry of Agriculture, BGI Research
    University of Chinese Academy of Sciences)

  • Jianmu Su

    (South China Agricultural University)

  • Xinyue Luo

    (Ministry of Agriculture, BGI Research
    University of Chinese Academy of Sciences)

  • Xiaohuan Jin

    (Ministry of Agriculture, BGI Research)

  • Jiao Zhao

    (Ministry of Agriculture, BGI Research)

  • Hong Wu

    (South China Agricultural University)

  • E-Hu Liu

    (Nanjing University of Chinese Medicine)

  • Huan Liu

    (Ministry of Agriculture, BGI Research)

Abstract

Citrus reticulata cv. Chachiensis (CRC) is an important medicinal plant, its dried mature peels named “Guangchenpi”, has been used as a traditional Chinese medicine to treat cough, indigestion, and lung diseases for several hundred years. However, the biosynthesis of the crucial natural products polymethoxylated flavonoids (PMFs) in CRC remains unclear. Here, we report a chromosome-scale genome assembly of CRC with the size of 314.96 Mb and a contig N50 of 16.22 Mb. Using multi-omics resources, we discover a putative caffeic acid O-methyltransferase (CcOMT1) that can transfer a methyl group to the 3-hydroxyl of natsudaidain to form 3,5,6,7,8,3’,4’-heptamethoxyflavone (HPMF). Based on transient overexpression and virus-induced gene silencing experiments, we propose that CcOMT1 is a candidate enzyme in HPMF biosynthesis. In addition, a potential gene regulatory network associated with PMF biosynthesis is identified. This study provides insights into PMF biosynthesis and may assist future research on mining genes for the biosynthesis of plant-based medicines.

Suggested Citation

  • Jiawen Wen & Yayu Wang & Xu Lu & Huimin Pan & Dian Jin & Jialing Wen & Canzhi Jin & Sunil Kumar Sahu & Jianmu Su & Xinyue Luo & Xiaohuan Jin & Jiao Zhao & Hong Wu & E-Hu Liu & Huan Liu, 2024. "An integrated multi-omics approach reveals polymethoxylated flavonoid biosynthesis in Citrus reticulata cv. Chachiensis," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48235-y
    DOI: 10.1038/s41467-024-48235-y
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    References listed on IDEAS

    as
    1. Guohong Albert Wu & Javier Terol & Victoria Ibanez & Antonio López-García & Estela Pérez-Román & Carles Borredá & Concha Domingo & Francisco R. Tadeo & Jose Carbonell-Caballero & Roberto Alonso & Fran, 2018. "Genomics of the origin and evolution of Citrus," Nature, Nature, vol. 554(7692), pages 311-316, February.
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