IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-48056-z.html
   My bibliography  Save this article

Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set

Author

Listed:
  • Tomoshige Fujino

    (Nagoya University)

  • Ryogo Sonoda

    (Nagoya University)

  • Taito Higashinagata

    (Nagoya University)

  • Emi Mishiro-Sato

    (Nagoya University)

  • Keiko Kano

    (Nagoya University)

  • Hiroshi Murakami

    (Nagoya University
    Nagoya University)

Abstract

The Ser/Leu-swapped genetic code can act as a genetic firewall, mitigating biohazard risks arising from horizontal gene transfer in genetically modified organisms. Our prior work demonstrated the orthogonality of this swapped code to the standard genetic code using a cell-free translation system comprised of 21 in vitro transcribed tRNAs. In this study, to advance this system for protein engineering, we introduce a natural/in vitro transcribed-hybrid tRNA set. This set combines natural tRNAs from Escherichia coli (excluding Ser, Leu, and Tyr) and in vitro transcribed tRNAs, encompassing anticodon-swapped tRNASerGAG and tRNALeuGGA. This approach reduces the number of in vitro transcribed tRNAs required from 21 to only 4. In this optimized system, the production of a model protein, superfolder green fluorescent protein, increases to 3.5-fold. With this hybrid tRNA set, the Ser/Leu-swapped cell-free translation system will stand as a potent tool for protein production with reduced biohazard concerns in future biological endeavors.

Suggested Citation

  • Tomoshige Fujino & Ryogo Sonoda & Taito Higashinagata & Emi Mishiro-Sato & Keiko Kano & Hiroshi Murakami, 2024. "Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48056-z
    DOI: 10.1038/s41467-024-48056-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-48056-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-48056-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Akos Nyerges & Svenja Vinke & Regan Flynn & Siân V. Owen & Eleanor A. Rand & Bogdan Budnik & Eric Keen & Kamesh Narasimhan & Jorge A. Marchand & Maximilien Baas-Thomas & Min Liu & Kangming Chen & Anus, 2023. "A swapped genetic code prevents viral infections and gene transfer," Nature, Nature, vol. 615(7953), pages 720-727, March.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Tiantian Chang & Weichao Ding & Shirui Yan & Yun Wang & Haoling Zhang & Yu Zhang & Zhi Ping & Huiming Zhang & Yijian Huang & Jiahui Zhang & Dan Wang & Wenwei Zhang & Xun Xu & Yue Shen & Xian Fu, 2023. "A robust yeast biocontainment system with two-layered regulation switch dependent on unnatural amino acid," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Kasimir Kienbeck & Lukas Malfertheiner & Susann Zelger-Paulus & Silke Johannsen & Christian Mering & Roland K. O. Sigel, 2024. "Identification of HDV-like theta ribozymes involved in tRNA-based recoding of gut bacteriophages," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48056-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.