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Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast

Author

Listed:
  • Lihui Jiang

    (Yunnan Agricultural University)

  • Xiaoyan Zhang

    (Yunnan Agricultural University)

  • Yiting Zhao

    (Yunnan Agricultural University
    Shanxi Agricultural University/Shanxi Academy of Agricultural Sciences. The Industrial Crop Institute)

  • Haiyan Zhu

    (Yunnan Agricultural University)

  • Qijing Fu

    (Yunnan Agricultural University)

  • Xinqi Lu

    (Yunnan Agricultural University)

  • Wuying Huang

    (Yunnan Agricultural University)

  • Xinyue Yang

    (Yunnan Agricultural University)

  • Xuan Zhou

    (Yunnan Agricultural University)

  • Lixia Wu

    (Yunnan Agricultural University)

  • Ao Yang

    (Yunnan Agricultural University)

  • Xie He

    (Yunnan Agricultural University)

  • Man Dong

    (Yunnan Agricultural University)

  • Ziai Peng

    (Yunnan Agricultural University)

  • Jing Yang

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

  • Liwei Guo

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

  • Jiancheng Wen

    (Yunnan Agricultural University)

  • Huichuan Huang

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

  • Yong Xie

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

  • Shusheng Zhu

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

  • Chengyun Li

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

  • Xiahong He

    (Southwest Forestry University)

  • Youyong Zhu

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

  • Jiří Friml

    (Institute of Science and Technology Austria (IST Austria))

  • Yunlong Du

    (Yunnan Agricultural University
    Yunnan Agricultural University
    Yunnan Agricultural University)

Abstract

Phytoalexin sakuranetin functions in resistance against rice blast. However, the mechanisms underlying the effects of sakuranetin remains elusive. Here, we report that rice lines expressing resistance (R) genes were found to contain high levels of sakuranetin, which correlates with attenuated endocytic trafficking of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates the endocytosis of various PM proteins and the fungal effector PWL2. Moreover, accumulation of the avirulence protein AvrCO39, resulting from uptake into rice cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin. Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin is sufficient to convey resistance against rice blast. Our data reveals a mechanism of rice against M. oryzae by increasing sakuranetin levels and repressing the CME of pathogen effectors, which is distinct from the action of many R genes that mainly function by modulating transcription.

Suggested Citation

  • Lihui Jiang & Xiaoyan Zhang & Yiting Zhao & Haiyan Zhu & Qijing Fu & Xinqi Lu & Wuying Huang & Xinyue Yang & Xuan Zhou & Lixia Wu & Ao Yang & Xie He & Man Dong & Ziai Peng & Jing Yang & Liwei Guo & Ji, 2024. "Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47746-y
    DOI: 10.1038/s41467-024-47746-y
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    References listed on IDEAS

    as
    1. Wim Dejonghe & Sabine Kuenen & Evelien Mylle & Mina Vasileva & Olivier Keech & Corrado Viotti & Jef Swerts & Matyáš Fendrych & Fausto Andres Ortiz-Morea & Kiril Mishev & Simon Delang & Stefan Scholl &, 2016. "Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification," Nature Communications, Nature, vol. 7(1), pages 1-12, September.
    2. Zi-Wei Yan & Fang-Yan Chen & Xian Zhang & Wen-Juan Cai & Chun-Yu Chen & Jie Liu & Man-Ni Wu & Ning-Jing Liu & Bin Ma & Mu-Yang Wang & Dai-Yin Chao & Cai-Ji Gao & Ying-Bo Mao, 2023. "Endocytosis-mediated entry of a caterpillar effector into plants is countered by Jasmonate," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
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