IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-47717-3.html
   My bibliography  Save this article

Methylation of ESCRT-III components regulates the timing of cytokinetic abscission

Author

Listed:
  • Aurélie Richard

    (UMR7126 Epigenetics and Cell Fate)

  • Jérémy Berthelet

    (UMR7126 Epigenetics and Cell Fate)

  • Delphine Judith

    (Institut Cochin)

  • Tamara Advedissian

    (Institut Pasteur, Université Paris Cité, CNRS UMR3691, Membrane Traffic and Cell Division Unit)

  • Javier Espadas

    (University of Geneva)

  • Guillaume Jannot

    (UMR7126 Epigenetics and Cell Fate)

  • Angélique Amo

    (UMR7126 Epigenetics and Cell Fate)

  • Damarys Loew

    (PSL Research University, Centre de Recherche, CurieCoreTech Mass Spectrometry Proteomics)

  • Berangere Lombard

    (PSL Research University, Centre de Recherche, CurieCoreTech Mass Spectrometry Proteomics)

  • Alexandre G. Casanova

    (Institute for Advanced Biosciences)

  • Nicolas Reynoird

    (Institute for Advanced Biosciences)

  • Aurélien Roux

    (University of Geneva)

  • Clarisse Berlioz-Torrent

    (Institut Cochin)

  • Arnaud Echard

    (Institut Pasteur, Université Paris Cité, CNRS UMR3691, Membrane Traffic and Cell Division Unit)

  • Jonathan B. Weitzman

    (UMR7126 Epigenetics and Cell Fate)

  • Souhila Medjkane

    (UMR7126 Epigenetics and Cell Fate)

Abstract

Abscission is the final stage of cytokinesis, which cleaves the intercellular bridge (ICB) connecting two daughter cells. Abscission requires tight control of the recruitment and polymerization of the Endosomal Protein Complex Required for Transport-III (ESCRT-III) components. We explore the role of post-translational modifications in regulating ESCRT dynamics. We discover that SMYD2 methylates the lysine 6 residue of human CHMP2B, a key ESCRT-III component, at the ICB, impacting the dynamic relocation of CHMP2B to sites of abscission. SMYD2 loss-of-function (genetically or pharmacologically) causes CHMP2B hypomethylation, delayed CHMP2B polymerization and delayed abscission. This is phenocopied by CHMP2B lysine 6 mutants that cannot be methylated. Conversely, SMYD2 gain-of-function causes CHMP2B hypermethylation and accelerated abscission, specifically in cells undergoing cytokinetic challenges, thereby bypassing the abscission checkpoint. Additional experiments highlight the importance of CHMP2B methylation beyond cytokinesis, namely during ESCRT-III-mediated HIV-1 budding. We propose that lysine methylation signaling fine-tunes the ESCRT-III machinery to regulate the timing of cytokinetic abscission and other ESCRT-III dependent functions.

Suggested Citation

  • Aurélie Richard & Jérémy Berthelet & Delphine Judith & Tamara Advedissian & Javier Espadas & Guillaume Jannot & Angélique Amo & Damarys Loew & Berangere Lombard & Alexandre G. Casanova & Nicolas Reyno, 2024. "Methylation of ESCRT-III components regulates the timing of cytokinetic abscission," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47717-3
    DOI: 10.1038/s41467-024-47717-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-47717-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-47717-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Cyril Addi & Adrien Presle & Stéphane Frémont & Frédérique Cuvelier & Murielle Rocancourt & Florine Milin & Sandrine Schmutz & Julia Chamot-Rooke & Thibaut Douché & Magalie Duchateau & Quentin Giai Gi, 2020. "The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    2. Aurélie Bertin & Nicola Franceschi & Eugenio Mora & Sourav Maity & Maryam Alqabandi & Nolwen Miguet & Aurélie Cicco & Wouter H. Roos & Stéphanie Mangenot & Winfried Weissenhorn & Patricia Bassereau, 2020. "Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Nebojsa Jukic & Alma P. Perrino & Frédéric Humbert & Aurélien Roux & Simon Scheuring, 2022. "Snf7 spirals sense and alter membrane curvature," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. B. Vijayalakshmi Ayyar & Khalil Ettayebi & Wilhelm Salmen & Umesh C. Karandikar & Frederick H. Neill & Victoria R. Tenge & Sue E. Crawford & Erhard Bieberich & B. V. Venkataram Prasad & Robert L. Atma, 2023. "CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47717-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.