IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-47412-3.html
   My bibliography  Save this article

Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin

Author

Listed:
  • Jess Tyson

    (Queen’s Medical Centre
    MediCity)

  • Paul Radford

    (Queen’s Medical Centre)

  • Carey Lambert

    (Queen’s Medical Centre
    University of Nottingham)

  • Rob Till

    (Queen’s Medical Centre
    University of Nottingham)

  • Simona G. Huwiler

    (CH-)

  • Andrew L. Lovering

    (University of Birmingham)

  • R. Elizabeth Sockett

    (Queen’s Medical Centre)

Abstract

The bacterium Bdellovibrio bacteriovorus is a predator of other Gram-negative bacteria. The predator invades the prey’s periplasm and modifies the prey’s cell wall, forming a rounded killed prey, or bdelloplast, containing a live B. bacteriovorus. Redundancy in adhesive processes makes invasive mutants rare. Here, we identify a MIDAS adhesin family protein, Bd0875, that is expressed at the predator-prey invasive junction and is important for successful invasion of prey. A mutant strain lacking bd0875 is still able to form round, dead bdelloplasts; however, 10% of the bdelloplasts do not contain B. bacteriovorus, indicative of an invasion defect. Bd0875 activity requires the conserved MIDAS motif, which is linked to catch-and-release activity of MIDAS proteins in other organisms. A proteomic analysis shows that the uninvaded bdelloplasts contain B. bacteriovorus proteins, which are likely secreted into the prey by the Δbd0875 predator during an abortive invasion period. Thus, secretion of proteins into the prey seems to be sufficient for prey killing, even in the absence of a live predator inside the prey periplasm.

Suggested Citation

  • Jess Tyson & Paul Radford & Carey Lambert & Rob Till & Simona G. Huwiler & Andrew L. Lovering & R. Elizabeth Sockett, 2024. "Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47412-3
    DOI: 10.1038/s41467-024-47412-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-47412-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-47412-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Emma J. Banks & Mauricio Valdivia-Delgado & Jacob Biboy & Amber Wilson & Ian T. Cadby & Waldemar Vollmer & Carey Lambert & Andrew L. Lovering & R. Elizabeth Sockett, 2022. "Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Sebastian Pöhl & Giacomo Giacomelli & Fabian M. Meyer & Volker Kleeberg & Eli J. Cohen & Jacob Biboy & Julia Rosum & Timo Glatter & Waldemar Vollmer & Muriel C. F. Teeseling & Johann Heider & Marc Bra, 2024. "An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47412-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.