Author
Listed:
- Pranay Shah
(MRC Laboratory of Molecular Biology)
- Ross Hill
(MRC Laboratory of Molecular Biology)
- Camille Dion
(MRC Laboratory of Medical Sciences
Imperial College London)
- Stephen J. Clark
(Altos Labs
The Babraham Institute)
- Abdulkadir Abakir
(Altos Labs
The Babraham Institute)
- Jeroen Willems
(Royal Netherlands Academy of Arts and Sciences (KNAW))
- Mark J. Arends
(Cancer Research UK Edinburgh Centre)
- Juan I. Garaycoechea
(Royal Netherlands Academy of Arts and Sciences (KNAW))
- Harry G. Leitch
(MRC Laboratory of Medical Sciences
Imperial College London)
- Wolf Reik
(Altos Labs
The Babraham Institute)
- Gerry P. Crossan
(MRC Laboratory of Molecular Biology)
Abstract
Mutations in DNA damage response (DDR) factors are associated with human infertility, which affects up to 15% of the population. The DDR is required during germ cell development and meiosis. One pathway implicated in human fertility is DNA translesion synthesis (TLS), which allows replication impediments to be bypassed. We find that TLS is essential for pre-meiotic germ cell development in the embryo. Loss of the central TLS component, REV1, significantly inhibits the induction of human PGC-like cells (hPGCLCs). This is recapitulated in mice, where deficiencies in TLS initiation (Rev1-/- or PcnaK164R/K164R) or extension (Rev7 -/-) result in a > 150-fold reduction in the number of primordial germ cells (PGCs) and complete sterility. In contrast, the absence of TLS does not impact the growth, function, or homeostasis of somatic tissues. Surprisingly, we find a complete failure in both activation of the germ cell transcriptional program and in DNA demethylation, a critical step in germline epigenetic reprogramming. Our findings show that for normal fertility, DNA repair is required not only for meiotic recombination but for progression through the earliest stages of germ cell development in mammals.
Suggested Citation
Pranay Shah & Ross Hill & Camille Dion & Stephen J. Clark & Abdulkadir Abakir & Jeroen Willems & Mark J. Arends & Juan I. Garaycoechea & Harry G. Leitch & Wolf Reik & Gerry P. Crossan, 2024.
"Primordial germ cell DNA demethylation and development require DNA translesion synthesis,"
Nature Communications, Nature, vol. 15(1), pages 1-20, December.
Handle:
RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47219-2
DOI: 10.1038/s41467-024-47219-2
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