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Microevolution, reinfection and highly complex genomic diversity in patients with sequential isolates of Mycobacterium abscessus

Author

Listed:
  • Sergio Buenestado-Serrano

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM)
    Universidad de Alcalá)

  • Miguel Martínez-Lirola

    (Hospital Universitario Torrecárdenas)

  • Marta Herranz-Martín

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM))

  • Jaime Esteban

    (Hospital Universitario La Fundación Jiménez Díaz
    Instituto de Salud Carlos III)

  • Antonio Broncano-Lavado

    (Hospital Universitario La Fundación Jiménez Díaz)

  • Andrea Molero-Salinas

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM))

  • Amadeo Sanz-Pérez

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM))

  • Jesús Blázquez

    (Campus de la Universidad Autónoma-Cantoblanco)

  • Alba Ruedas-López

    (Hospital Universitario 12 de Octubre)

  • Carlos Toro

    (Hospital Universitario La Paz - IdiPAZ)

  • Paula López-Roa

    (Hospital Universitario 12 de Octubre)

  • Diego Domingo

    (Instituto de Investigación Sanitaria, Hospital Universitario La Princesa)

  • Ester Zamarrón

    (Hospital Universitario La Paz -IdiPAZ)

  • María Jesús Ruiz Serrano

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM)
    Instituto de Salud Carlos III)

  • Patricia Muñoz

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM)
    Instituto de Salud Carlos III
    Universidad Complutense)

  • Laura Pérez-Lago

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM))

  • Darío García de Viedma

    (Hospital General Universitario Gregorio Marañón
    Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM)
    Instituto de Salud Carlos III)

Abstract

Mycobacterium abscessus is an opportunistic, extensively drug-resistant non-tuberculous mycobacterium. Few genomic studies consider its diversity in persistent infections. Our aim was to characterize microevolution/reinfection events in persistent infections. Fifty-three sequential isolates from 14 patients were sequenced to determine SNV-based distances, assign resistance mutations and characterize plasmids. Genomic analysis revealed 12 persistent cases (0-13 differential SNVs), one reinfection (15,956 SNVs) and one very complex case (23 sequential isolates over 192 months), in which a first period of persistence (58 months) involving the same genotype 1 was followed by identification of a genotype 2 (76 SNVs) in 6 additional alternating isolates; additionally, ten transient genotypes (88-243 SNVs) were found. A macrolide resistance mutation was identified from the second isolate. Despite high diversity, the genotypes shared a common phylogenetic ancestor and some coexisted in the same specimens. Genomic analysis is required to access the true intra-patient complexity behind persistent infections involving M. abscessus.

Suggested Citation

  • Sergio Buenestado-Serrano & Miguel Martínez-Lirola & Marta Herranz-Martín & Jaime Esteban & Antonio Broncano-Lavado & Andrea Molero-Salinas & Amadeo Sanz-Pérez & Jesús Blázquez & Alba Ruedas-López & C, 2024. "Microevolution, reinfection and highly complex genomic diversity in patients with sequential isolates of Mycobacterium abscessus," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46552-w
    DOI: 10.1038/s41467-024-46552-w
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    References listed on IDEAS

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    1. Ryan A. Bronson & Chhavi Gupta & Abigail L. Manson & Jan A. Nguyen & Asli Bahadirli-Talbott & Nicole M. Parrish & Ashlee M. Earl & Keira A. Cohen, 2021. "Global phylogenomic analyses of Mycobacterium abscessus provide context for non cystic fibrosis infections and the evolution of antibiotic resistance," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
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