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Mosquito midgut stem cell cellular defense response limits Plasmodium parasite infection

Author

Listed:
  • Ana-Beatriz F. Barletta

    (National Institutes of Health)

  • Jamie C. Smith

    (National Institutes of Health)

  • Emily Burkart

    (National Institutes of Health)

  • Simon Bondarenko

    (Virginia Polytechnic Institute and State University)

  • Igor V. Sharakhov

    (Virginia Polytechnic Institute and State University)

  • Frank Criscione

    (National Institutes of Health)

  • David O’Brochta

    (Institute for Bioscience and Biotechnology Research and Department of Entomology University of Maryland-College Park)

  • Carolina Barillas-Mury

    (National Institutes of Health)

Abstract

A novel cellular response of midgut progenitors (stem cells and enteroblasts) to Plasmodium berghei infection was investigated in Anopheles stephensi. The presence of developing oocysts triggers proliferation of midgut progenitors that is modulated by the Jak/STAT pathway and is proportional to the number of oocysts on individual midguts. The percentage of parasites in direct contact with enteroblasts increases over time, as progenitors proliferate. Silencing components of key signaling pathways through RNA interference (RNAi) that enhance proliferation of progenitor cells significantly decreased oocyst numbers, while limiting proliferation of progenitors increased oocyst survival. Live imaging revealed that enteroblasts interact directly with oocysts and eliminate them. Midgut progenitors sense the presence of Plasmodium oocysts and mount a cellular defense response that involves extensive proliferation and tissue remodeling, followed by oocysts lysis and phagocytosis of parasite remnants by enteroblasts.

Suggested Citation

  • Ana-Beatriz F. Barletta & Jamie C. Smith & Emily Burkart & Simon Bondarenko & Igor V. Sharakhov & Frank Criscione & David O’Brochta & Carolina Barillas-Mury, 2024. "Mosquito midgut stem cell cellular defense response limits Plasmodium parasite infection," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45550-2
    DOI: 10.1038/s41467-024-45550-2
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    References listed on IDEAS

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    1. Daniel Jun-Kit Hu & Jina Yun & Justin Elstrott & Heinrich Jasper, 2021. "Non-canonical Wnt signaling promotes directed migration of intestinal stem cells to sites of injury," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    2. Benjamin Ohlstein & Allan Spradling, 2006. "The adult Drosophila posterior midgut is maintained by pluripotent stem cells," Nature, Nature, vol. 439(7075), pages 470-474, January.
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