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In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells

Author

Listed:
  • Yusuke Seto

    (Kyoto University
    Kyoto University)

  • Ryoma Ogihara

    (Kyoto University)

  • Kaori Takizawa

    (Kyoto University)

  • Mototsugu Eiraku

    (Kyoto University
    Kyoto University
    Kyoto University)

Abstract

Early patterning of neural crest cells (NCCs) in the craniofacial primordium is important for subsequent development of proper craniofacial structures. However, because of the complexity of the environment of developing tissues, surveying the early specification and patterning of NCCs is difficult. In this study, we develop a simplified in vitro 3D model using human pluripotent stem cells to analyze the early stages of facial development. In this model, cranial NCC-like cells spontaneously differentiate from neural plate border-like cells into maxillary arch-like mesenchyme after a long-term culture. Upon the addition of EDN1 and BMP4, these aggregates are converted into a mandibular arch-like state. Furthermore, temporary treatment with EDN1 and BMP4 induces the formation of spatially separated domains expressing mandibular and maxillary arch markers within a single aggregate. These results suggest that this in vitro model is useful for determining the mechanisms underlying cell fate specification and patterning during early facial development.

Suggested Citation

  • Yusuke Seto & Ryoma Ogihara & Kaori Takizawa & Mototsugu Eiraku, 2024. "In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45285-0
    DOI: 10.1038/s41467-024-45285-0
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    References listed on IDEAS

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    1. Hirotaka Tao & Min Zhu & Kimberly Lau & Owen K. W. Whitley & Mohammad Samani & Xiao Xiao & Xiao Xiao Chen & Noah A. Hahn & Weifan Liu & Megan Valencia & Min Wu & Xian Wang & Kelli D. Fenelon & Clariss, 2019. "Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch," Nature Communications, Nature, vol. 10(1), pages 1-18, December.
    2. Christopher Bono & Yang Liu & Alexander Ferrena & Aneesa Valentine & Deyou Zheng & Bernice E. Morrow, 2023. "Single-cell transcriptomics uncovers a non-autonomous Tbx1-dependent genetic program controlling cardiac neural crest cell development," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
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