IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-44889-w.html
   My bibliography  Save this article

Applying valency-based immuno-selection to generate broadly cross-reactive antibodies against influenza hemagglutinins

Author

Listed:
  • Daniëla Maria Hinke

    (University of Oslo
    University of Oslo and Oslo University Hospital)

  • Ane Marie Anderson

    (University of Oslo
    University of Oslo and Oslo University Hospital)

  • Kirankumar Katta

    (University of Oslo and Oslo University Hospital)

  • Marlene Fyrstenberg Laursen

    (University of Oslo and Oslo University Hospital)

  • Demo Yemane Tesfaye

    (University of Oslo and Oslo University Hospital)

  • Ina Charlotta Werninghaus

    (University of Oslo and Oslo University Hospital)

  • Davide Angeletti

    (University of Gothenburg)

  • Gunnveig Grødeland

    (University of Oslo
    University of Oslo and Oslo University Hospital)

  • Bjarne Bogen

    (University of Oslo
    University of Oslo and Oslo University Hospital)

  • Ranveig Braathen

    (University of Oslo
    University of Oslo and Oslo University Hospital)

Abstract

Conserved epitopes shared between virus subtypes are often subdominant, making it difficult to induce broadly reactive antibodies by immunization. Here, we generate a plasmid DNA mix vaccine that encodes protein heterodimers with sixteen different influenza A virus hemagglutinins (HA) representing all HA subtypes except H1 (group 1) and H7 (group 2). Each single heterodimer expresses two different HA subtypes and is targeted to MHC class II on antigen presenting cells (APC). Female mice immunized with the plasmid mix produce antibodies not only against the 16 HA subtypes, but also against non-included H1 and H7. We demonstrate that individual antibody molecules cross-react between different HAs. Furthermore, the mix vaccine induces T cell responses to conserved HA epitopes. Immunized mice are partially protected against H1 viruses. The results show that application of valency-based immuno-selection to diversified antigens can be used to direct antibody responses towards conserved (subdominant) epitopes on viral antigens.

Suggested Citation

  • Daniëla Maria Hinke & Ane Marie Anderson & Kirankumar Katta & Marlene Fyrstenberg Laursen & Demo Yemane Tesfaye & Ina Charlotta Werninghaus & Davide Angeletti & Gunnveig Grødeland & Bjarne Bogen & Ran, 2024. "Applying valency-based immuno-selection to generate broadly cross-reactive antibodies against influenza hemagglutinins," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-44889-w
    DOI: 10.1038/s41467-024-44889-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-44889-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-44889-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Michael B. Doud & Juhye M. Lee & Jesse D. Bloom, 2018. "How single mutations affect viral escape from broad and narrow antibodies to H1 influenza hemagglutinin," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    2. Jenna J. Guthmiller & Julianna Han & Henry A. Utset & Lei Li & Linda Yu-Ling Lan & Carole Henry & Christopher T. Stamper & Meagan McMahon & George O’Dell & Monica L. Fernández-Quintero & Alec W. Freyn, 2022. "Broadly neutralizing antibodies target a haemagglutinin anchor epitope," Nature, Nature, vol. 602(7896), pages 314-320, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Kemin Tan & Junjian Chen & Yu Kaku & Yi Wang & Luke Donius & Rafiq Ahmad Khan & Xiaolong Li & Hannah Richter & Michael S. Seaman & Thomas Walz & Wonmuk Hwang & Ellis L. Reinherz & Mikyung Kim, 2023. "Inadequate structural constraint on Fab approach rather than paratope elicitation limits HIV-1 MPER vaccine utility," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Ling Huang & Wei Tang & Lina He & Mengke Li & Xian Lin & Ao Hu & Xindi Huang & Zhouyu Wu & Zhiyong Wu & Shiyun Chen & Yangbo Hu, 2024. "Engineered probiotic Escherichia coli elicits immediate and long-term protection against influenza A virus in mice," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-44889-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.