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Origin of the multi-phasic quenching dynamics in the BLUF domains across the species

Author

Listed:
  • Yalin Zhou

    (Shanghai Jiao Tong University)

  • Siwei Tang

    (Shanghai Jiao Tong University)

  • Zijing Chen

    (Shanghai Jiao Tong University)

  • Zhongneng Zhou

    (Shanghai Jiao Tong University)

  • Jiulong Huang

    (Shanghai Jiao Tong University)

  • Xiu-Wen Kang

    (Shanghai Jiao Tong University)

  • Shuhua Zou

    (Shanghai Jiao Tong University)

  • Bingyao Wang

    (Shanghai Jiao Tong University)

  • Tianyi Zhang

    (Shanghai Jiao Tong University)

  • Bei Ding

    (Shanghai Jiao Tong University)

  • Dongping Zhong

    (Shanghai Jiao Tong University
    The Ohio State University)

Abstract

Blue light using flavin (BLUF) photoreceptors respond to light via one of nature’s smallest photo-switching domains. Upon photo-activation, the flavin cofactor in the BLUF domain exhibits multi-phasic dynamics, quenched by a proton-coupled electron transfer reaction involving the conserved Tyr and Gln. The dynamic behavior varies drastically across different species, the origin of which remains controversial. Here, we incorporate site-specific fluorinated Trp into three BLUF proteins, i.e., AppA, OaPAC and SyPixD, and characterize the percentages for the Wout, WinNHin and WinNHout conformations using 19F nuclear magnetic resonance spectroscopy. Using femtosecond spectroscopy, we identify that one key WinNHin conformation can introduce a branching one-step proton transfer in AppA and a two-step proton transfer in OaPAC and SyPixD. Correlating the flavin quenching dynamics with the active-site structural heterogeneity, we conclude that the quenching rate is determined by the percentage of WinNHin, which encodes a Tyr-Gln configuration that is not conducive to proton transfer.

Suggested Citation

  • Yalin Zhou & Siwei Tang & Zijing Chen & Zhongneng Zhou & Jiulong Huang & Xiu-Wen Kang & Shuhua Zou & Bingyao Wang & Tianyi Zhang & Bei Ding & Dongping Zhong, 2024. "Origin of the multi-phasic quenching dynamics in the BLUF domains across the species," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44565-5
    DOI: 10.1038/s41467-023-44565-5
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    1. Mineo Iseki & Shigeru Matsunaga & Akio Murakami & Kaoru Ohno & Kiyoshi Shiga & Kazuichi Yoshida & Michizo Sugai & Tetsuo Takahashi & Terumitsu Hori & Masakatsu Watanabe, 2002. "A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis," Nature, Nature, vol. 415(6875), pages 1047-1051, February.
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