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Mammalian cell growth characterisation by a non-invasive plate reader assay

Author

Listed:
  • Alice Grob

    (Imperial College London
    Imperial College London)

  • Chiara Enrico Bena

    (Italian Institute for Genomic Medicine
    Université Paris-Saclay (INRAE), AgroParisTech, Micalis Institute)

  • Roberto Di Blasi

    (Imperial College London
    Imperial College London)

  • Daniele Pessina

    (Imperial College London)

  • Matthew Sood

    (Imperial College London)

  • Zhou Yunyue

    (Imperial College London)

  • Carla Bosia

    (Italian Institute for Genomic Medicine
    Politecnico di Torino)

  • Mark Isalan

    (Imperial College London
    Imperial College London)

  • Francesca Ceroni

    (Imperial College London
    Imperial College London)

Abstract

Automated and non-invasive mammalian cell analysis is currently lagging behind due to a lack of methods suitable for a variety of cell lines and applications. Here, we report the development of a high throughput non-invasive method for tracking mammalian cell growth and performance based on plate reader measurements. We show the method to be suitable for both suspension and adhesion cell lines, and we demonstrate it can be adopted when cells are grown under different environmental conditions. We establish that the method is suitable to inform on effective drug treatments to be used depending on the cell line considered, and that it can support characterisation of engineered mammalian cells over time. This work provides the scientific community with an innovative approach to mammalian cell screening, also contributing to the current efforts towards high throughput and automated mammalian cell engineering.

Suggested Citation

  • Alice Grob & Chiara Enrico Bena & Roberto Di Blasi & Daniele Pessina & Matthew Sood & Zhou Yunyue & Carla Bosia & Mark Isalan & Francesca Ceroni, 2024. "Mammalian cell growth characterisation by a non-invasive plate reader assay," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44396-4
    DOI: 10.1038/s41467-023-44396-4
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