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Serine peptidase Vpr forms enzymatically active fibrils outside Bacillus bacteria revealed by cryo-EM

Author

Listed:
  • Yijia Cheng

    (Shanghai Jiao Tong University)

  • Jianting Han

    (Shanghai Jiao Tong University)

  • Meinai Song

    (Shanghai Jiao Tong University)

  • Shuqin Zhang

    (Shanghai Jiao Tong University)

  • Qin Cao

    (Shanghai Jiao Tong University)

Abstract

Bacteria develop a variety of extracellular fibrous structures crucial for their survival, such as flagella and pili. In this study, we use cryo-EM to identify protein fibrils surrounding lab-cultured Bacillus amyloiquefaciens and discover an unreported fibril species in addition to the flagellar fibrils. These previously unknown fibrils are composed of Vpr, an extracellular serine peptidase. We find that Vpr assembles into fibrils in an enzymatically active form, potentially representing a strategy of enriching Vpr activities around bacterial cells. Vpr fibrils are also observed under other culture conditions and around other Bacillus bacteria, such as Bacillus subtilis, which may suggest a general mechanism across all Bacillus bacterial groups. Taken together, our study reveals fibrils outside the bacterial cell and sheds light on the physiological role of these extracellular fibrils.

Suggested Citation

  • Yijia Cheng & Jianting Han & Meinai Song & Shuqin Zhang & Qin Cao, 2023. "Serine peptidase Vpr forms enzymatically active fibrils outside Bacillus bacteria revealed by cryo-EM," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43359-z
    DOI: 10.1038/s41467-023-43359-z
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    References listed on IDEAS

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    1. Yi Xiao Jiang & Qin Cao & Michael R. Sawaya & Romany Abskharon & Peng Ge & Michael DeTure & Dennis W. Dickson & Janine Y. Fu & Rachel R. Ogorzalek Loo & Joseph A. Loo & David S. Eisenberg, 2022. "Amyloid fibrils in FTLD-TDP are composed of TMEM106B and not TDP-43," Nature, Nature, vol. 605(7909), pages 304-309, May.
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    Cited by:

    1. Yijia Cheng & Mark A. B. Kreutzberger & Jianting Han & Edward H. Egelman & Qin Cao, 2024. "Molecular architecture of the assembly of Bacillus spore coat protein GerQ revealed by cryo-EM," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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    1. Yijia Cheng & Mark A. B. Kreutzberger & Jianting Han & Edward H. Egelman & Qin Cao, 2024. "Molecular architecture of the assembly of Bacillus spore coat protein GerQ revealed by cryo-EM," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
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