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Engineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds

Author

Listed:
  • Ruirui Xu

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Weijao Zhang

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Xintong Xi

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Jiamin Chen

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Yang Wang

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Guocheng Du

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Jianghua Li

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Jian Chen

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

  • Zhen Kang

    (Jiangnan University
    Jiangnan University
    Jiangnan University)

Abstract

Sulfonation as one of the most important modification reactions in nature is essential for many biological macromolecules to function. Development of green sulfonate group donor regeneration systems to efficiently sulfonate compounds of interest is always attractive. Here, we design and engineer two different sulfonate group donor regeneration systems to boost the biosynthesis of sulfated compounds. First, we assemble three modules to construct a 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration system and demonstrate its applicability for living cells. After discovering adenosine 5’-phosphosulfate (APS) as another active sulfonate group donor, we engineer a more simplified APS regeneration system that couples specific sulfotransferase. Next, we develop a rapid indicating system for characterizing the activity of APS-mediated sulfotransferase to rapidly screen sulfotransferase variants with increased activity towards APS. Eventually, the active sulfonate group equivalent values of the APS regeneration systems towards trehalose and p-coumaric acid reach 3.26 and 4.03, respectively. The present PAPS and APS regeneration systems are environmentally friendly and applicable for scaling up the biomanufacturing of sulfated products.

Suggested Citation

  • Ruirui Xu & Weijao Zhang & Xintong Xi & Jiamin Chen & Yang Wang & Guocheng Du & Jianghua Li & Jian Chen & Zhen Kang, 2023. "Engineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43195-1
    DOI: 10.1038/s41467-023-43195-1
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    References listed on IDEAS

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    1. Yuda Chen & Shikai Jin & Mengxi Zhang & Yu Hu & Kuan-Lin Wu & Anna Chung & Shichao Wang & Zeru Tian & Yixian Wang & Peter G. Wolynes & Han Xiao, 2022. "Unleashing the potential of noncanonical amino acid biosynthesis to create cells with precision tyrosine sulfation," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
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