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HOP1 and HAP2 are conserved components of the meiosis-related machinery required for successful mating in Leishmania

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  • Carolina Moura Costa Catta-Preta

    (National Institutes of Health)

  • Tiago Rodrigues Ferreira

    (National Institutes of Health)

  • Kashinath Ghosh

    (National Institutes of Health)

  • Andrea Paun

    (National Institutes of Health)

  • David Sacks

    (National Institutes of Health)

Abstract

Whole genome analysis of Leishmania hybrids generated experimentally in sand flies supports a meiotic mechanism of genetic exchange, with Mendelian segregation of the nuclear genome. Here, we perform functional analyses through the generation of double drug-resistant hybrids in vitro and in vivo (during sand fly infections) to assess the importance of conserved meiosis-related genes in recombination and plasmogamy. We report that HOP1 and a HAP2-paralog (HAP2-2) are essential components of the Leishmania meiosis machinery and cell-to-cell fusion mechanism, respectively, since deletion of either gene in one or both parents significantly reduces or completely abrogates mating competence. These findings significantly advance our understanding of sexual reproduction in Leishmania, with likely relevance to other trypanosomatids, by formally demonstrating the involvement of a meiotic protein homolog and a distinct fusogen that mediates non-canonical, bilateral fusion in the hybridizing cells.

Suggested Citation

  • Carolina Moura Costa Catta-Preta & Tiago Rodrigues Ferreira & Kashinath Ghosh & Andrea Paun & David Sacks, 2023. "HOP1 and HAP2 are conserved components of the meiosis-related machinery required for successful mating in Leishmania," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42789-z
    DOI: 10.1038/s41467-023-42789-z
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