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Staphylococcus aureus sacculus mediates activities of M23 hydrolases

Author

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  • Alicja Razew

    (Universite Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
    International Institute of Molecular and Cell Biology in Warsaw
    Polish Academy of Sciences)

  • Cedric Laguri

    (Universite Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale)

  • Alicia Vallet

    (Universite Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale)

  • Catherine Bougault

    (Universite Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale)

  • Magdalena Kaus-Drobek

    (Polish Academy of Sciences)

  • Izabela Sabala

    (International Institute of Molecular and Cell Biology in Warsaw
    Polish Academy of Sciences)

  • Jean-Pierre Simorre

    (Universite Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale)

Abstract

Peptidoglycan, a gigadalton polymer, functions as the scaffold for bacterial cell walls and provides cell integrity. Peptidoglycan is remodelled by a large and diverse group of peptidoglycan hydrolases, which control bacterial cell growth and division. Over the years, many studies have focused on these enzymes, but knowledge on their action within peptidoglycan mesh from a molecular basis is scarce. Here, we provide structural insights into the interaction between short peptidoglycan fragments and the entire sacculus with two evolutionarily related peptidases of the M23 family, lysostaphin and LytM. Through nuclear magnetic resonance, mass spectrometry, information-driven modelling, site-directed mutagenesis and biochemical approaches, we propose a model in which peptidoglycan cross-linking affects the activity, selectivity and specificity of these two structurally related enzymes differently.

Suggested Citation

  • Alicja Razew & Cedric Laguri & Alicia Vallet & Catherine Bougault & Magdalena Kaus-Drobek & Izabela Sabala & Jean-Pierre Simorre, 2023. "Staphylococcus aureus sacculus mediates activities of M23 hydrolases," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42506-w
    DOI: 10.1038/s41467-023-42506-w
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    1. L. Pasquina-Lemonche & J. Burns & R. D. Turner & S. Kumar & R. Tank & N. Mullin & J. S. Wilson & B. Chakrabarti & P. A. Bullough & S. J. Foster & J. K. Hobbs, 2020. "The architecture of the Gram-positive bacterial cell wall," Nature, Nature, vol. 582(7811), pages 294-297, June.
    2. Bai-Lu Tang & Jie Yang & Xiu-Lan Chen & Peng Wang & Hui-Lin Zhao & Hai-Nan Su & Chun-Yang Li & Yang Yu & Shuai Zhong & Lei Wang & Ian Lidbury & Haitao Ding & Min Wang & Andrew McMinn & Xi-Ying Zhang &, 2020. "A predator-prey interaction between a marine Pseudoalteromonas sp. and Gram-positive bacteria," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
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