IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-40662-7.html
   My bibliography  Save this article

Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish

Author

Listed:
  • Juan P. García-López

    (Andres Bello University)

  • Alexandre Grimaldi

    (Institut Pasteur
    Institut Pasteur)

  • Zelin Chen

    (Chinese Academy of Sciences)

  • Claudio Meneses

    (Millennium Nucleus Development of Super Adaptable Plants (MN-SAP)
    Millennium Institute Center for Genome Regulation (CRG)
    Pontificia Universidad Católica de Chile
    Pontificia Universidad Católica de Chile)

  • Karina Bravo-Tello

    (Andres Bello University)

  • Erica Bresciani

    (National Human Genome Research Institute)

  • Alvaro Banderas

    (CNRS UMR168, Laboratoire Physico Chimie Curie)

  • Shawn M. Burgess

    (National Human Genome Research Institute)

  • Pedro P. Hernández

    (PSL Research University, INSERM U934/CNRS UMR3215, Development and Homeostasis of Mucosal Tissues Lab)

  • Carmen G. Feijoo

    (Andres Bello University)

Abstract

The current view of hematopoiesis considers leukocytes on a continuum with distinct developmental origins, and which exert non-overlapping functions. However, there is less known about the function and phenotype of ontogenetically distinct neutrophil populations. In this work, using a photoconvertible transgenic zebrafish line; Tg(mpx:Dendra2), we selectively label rostral blood island-derived and caudal hematopoietic tissue-derived neutrophils in vivo during steady state or upon injury. By comparing the migratory properties and single-cell expression profiles of both neutrophil populations at steady state we show that rostral neutrophils show higher csf3b expression and migration capacity than caudal neutrophils. Upon injury, both populations share a core transcriptional profile as well as subset-specific transcriptional signatures. Accordingly, both rostral and caudal neutrophils are recruited to the wound independently of their distance to the injury. While rostral neutrophils respond uniformly, caudal neutrophils respond heterogeneously. Collectively, our results reveal that co-existing neutrophils populations with ontogenically distinct origin display functional differences.

Suggested Citation

  • Juan P. García-López & Alexandre Grimaldi & Zelin Chen & Claudio Meneses & Karina Bravo-Tello & Erica Bresciani & Alvaro Banderas & Shawn M. Burgess & Pedro P. Hernández & Carmen G. Feijoo, 2023. "Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40662-7
    DOI: 10.1038/s41467-023-40662-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-40662-7
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-40662-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Sa Kan Yoo & Taylor W. Starnes & Qing Deng & Anna Huttenlocher, 2011. "Lyn is a redox sensor that mediates leukocyte wound attraction in vivo," Nature, Nature, vol. 480(7375), pages 109-112, December.
    2. Karima Kissa & Philippe Herbomel, 2010. "Blood stem cells emerge from aortic endothelium by a novel type of cell transition," Nature, Nature, vol. 464(7285), pages 112-115, March.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Stefanie Kirchberger & Mohamed R. Shoeb & Daria Lazic & Andrea Wenninger-Weinzierl & Kristin Fischer & Lisa E. Shaw & Filomena Nogueira & Fikret Rifatbegovic & Eva Bozsaky & Ruth Ladenstein & Bernd Bo, 2024. "Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Xiaoyi Cheng & Radwa Barakat & Giulia Pavani & Masuma Khatun Usha & Rodolfo Calderon & Elizabeth Snella & Abigail Gorden & Yudi Zhang & Paul Gadue & Deborah L. French & Karin S. Dorman & Antonella Fid, 2023. "Nod1-dependent NF-kB activation initiates hematopoietic stem cell specification in response to small Rho GTPases," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    2. Szimonetta Xénia Tamás & Benoit Thomas Roux & Boldizsár Vámosi & Fabian Gregor Dehne & Anna Török & László Fazekas & Balázs Enyedi, 2023. "A genetically encoded sensor for visualizing leukotriene B4 gradients in vivo," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Emi Murayama & Catherine Vivier & Anne Schmidt & Philippe Herbomel, 2023. "Alcam-a and Pdgfr-α are essential for the development of sclerotome-derived stromal cells that support hematopoiesis," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    4. Dorothee Bornhorst & Amulya V. Hejjaji & Lena Steuter & Nicole M. Woodhead & Paul Maier & Alessandra Gentile & Alice Alhajkadour & Octavia Santis Larrain & Michael Weber & Khrievono Kikhi & Stefan Gue, 2024. "The heart is a resident tissue for hematopoietic stem and progenitor cells in zebrafish," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    5. Yumin Liu & Linjuan Shi & Yifan Chen & Sifan Luo & Yuehang Chen & Hongtian Chen & Wenlang Lan & Xun Lu & Zhan Cao & Zehua Ye & Jinping Li & Bo Yu & Elaine Dzierzak & Zhuan Li, 2024. "Autophagy regulates the maturation of hematopoietic precursors in the embryo," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    6. Wen Hao Neo & Yiran Meng & Alba Rodriguez-Meira & Muhammad Z. H. Fadlullah & Christopher A. G. Booth & Emanuele Azzoni & Supat Thongjuea & Marella F. T. R. Bruijn & Sten Eirik W. Jacobsen & Adam J. Me, 2021. "Ezh2 is essential for the generation of functional yolk sac derived erythro-myeloid progenitors," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    7. Zaniah N. Gonzalez Galofre & Alastair M. Kilpatrick & Madalena Marques & Diana Sá da Bandeira & Telma Ventura & Mario Gomez Salazar & Léa Bouilleau & Yvan Marc & Ana B. Barbosa & Fiona Rossi & Mariana, 2024. "Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40662-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.