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Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish

Author

Listed:
  • Juan P. García-López

    (Andres Bello University)

  • Alexandre Grimaldi

    (Institut Pasteur
    Institut Pasteur)

  • Zelin Chen

    (Chinese Academy of Sciences)

  • Claudio Meneses

    (Millennium Nucleus Development of Super Adaptable Plants (MN-SAP)
    Millennium Institute Center for Genome Regulation (CRG)
    Pontificia Universidad Católica de Chile
    Pontificia Universidad Católica de Chile)

  • Karina Bravo-Tello

    (Andres Bello University)

  • Erica Bresciani

    (National Human Genome Research Institute)

  • Alvaro Banderas

    (CNRS UMR168, Laboratoire Physico Chimie Curie)

  • Shawn M. Burgess

    (National Human Genome Research Institute)

  • Pedro P. Hernández

    (PSL Research University, INSERM U934/CNRS UMR3215, Development and Homeostasis of Mucosal Tissues Lab)

  • Carmen G. Feijoo

    (Andres Bello University)

Abstract

The current view of hematopoiesis considers leukocytes on a continuum with distinct developmental origins, and which exert non-overlapping functions. However, there is less known about the function and phenotype of ontogenetically distinct neutrophil populations. In this work, using a photoconvertible transgenic zebrafish line; Tg(mpx:Dendra2), we selectively label rostral blood island-derived and caudal hematopoietic tissue-derived neutrophils in vivo during steady state or upon injury. By comparing the migratory properties and single-cell expression profiles of both neutrophil populations at steady state we show that rostral neutrophils show higher csf3b expression and migration capacity than caudal neutrophils. Upon injury, both populations share a core transcriptional profile as well as subset-specific transcriptional signatures. Accordingly, both rostral and caudal neutrophils are recruited to the wound independently of their distance to the injury. While rostral neutrophils respond uniformly, caudal neutrophils respond heterogeneously. Collectively, our results reveal that co-existing neutrophils populations with ontogenically distinct origin display functional differences.

Suggested Citation

  • Juan P. García-López & Alexandre Grimaldi & Zelin Chen & Claudio Meneses & Karina Bravo-Tello & Erica Bresciani & Alvaro Banderas & Shawn M. Burgess & Pedro P. Hernández & Carmen G. Feijoo, 2023. "Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40662-7
    DOI: 10.1038/s41467-023-40662-7
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    References listed on IDEAS

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    1. Karima Kissa & Philippe Herbomel, 2010. "Blood stem cells emerge from aortic endothelium by a novel type of cell transition," Nature, Nature, vol. 464(7285), pages 112-115, March.
    2. Sa Kan Yoo & Taylor W. Starnes & Qing Deng & Anna Huttenlocher, 2011. "Lyn is a redox sensor that mediates leukocyte wound attraction in vivo," Nature, Nature, vol. 480(7375), pages 109-112, December.
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    1. Stefanie Kirchberger & Mohamed R. Shoeb & Daria Lazic & Andrea Wenninger-Weinzierl & Kristin Fischer & Lisa E. Shaw & Filomena Nogueira & Fikret Rifatbegovic & Eva Bozsaky & Ruth Ladenstein & Bernd Bo, 2024. "Comparative transcriptomics coupled to developmental grading via transgenic zebrafish reporter strains identifies conserved features in neutrophil maturation," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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