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Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding

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  • Laurie Lannes

    (National Institutes of Health)

  • Christopher M. Furman

    (National Institutes of Health)

  • Alison B. Hickman

    (National Institutes of Health)

  • Fred Dyda

    (National Institutes of Health)

Abstract

The Hermes DNA transposon is a member of the eukaryotic hAT superfamily, and its transposase forms a ring-shaped tetramer of dimers. Our investigation, combining biochemical, crystallography and cryo-electron microscopy, and in-cell assays, shows that the full-length Hermes octamer extensively interacts with its transposon left-end through multiple BED domains of three Hermes protomers contributed by three dimers explaining the role of the unusual higher-order assembly. By contrast, the right-end is bound to no BED domains at all. Thus, this work supports a model in which Hermes multimerizes to gather enough BED domains to find its left-end among the abundant genomic DNA, facilitating the subsequent interaction with the right-end.

Suggested Citation

  • Laurie Lannes & Christopher M. Furman & Alison B. Hickman & Fred Dyda, 2023. "Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40210-3
    DOI: 10.1038/s41467-023-40210-3
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    References listed on IDEAS

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    1. Ivana Grabundzija & Alison B. Hickman & Fred Dyda, 2018. "Helraiser intermediates provide insight into the mechanism of eukaryotic replicative transposition," Nature Communications, Nature, vol. 9(1), pages 1-14, December.
    2. Chang Liu & Yang Yang & David G. Schatz, 2019. "Structures of a RAG-like transposase during cut-and-paste transposition," Nature, Nature, vol. 575(7783), pages 540-544, November.
    3. Christian Biémont & Cristina Vieira, 2006. "Junk DNA as an evolutionary force," Nature, Nature, vol. 443(7111), pages 521-524, October.
    4. Sherwin P. Montaño & Ying Z. Pigli & Phoebe A. Rice, 2012. "The Mu transpososome structure sheds light on DDE recombinase evolution," Nature, Nature, vol. 491(7424), pages 413-417, November.
    5. Liqin Zhou & Rupak Mitra & Peter W. Atkinson & Alison Burgess Hickman & Fred Dyda & Nancy L. Craig, 2004. "Transposition of hAT elements links transposable elements and V(D)J recombination," Nature, Nature, vol. 432(7020), pages 995-1001, December.
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    Cited by:

    1. Alison B. Hickman & Laurie Lannes & Christopher M. Furman & Christina Hong & Lidiya Franklin & Rodolfo Ghirlando & Arpita Ghosh & Wentian Luo & Parthena Konstantinidou & Hernán A. Lorenzi & Anne Grove, 2025. "Activity of the mammalian DNA transposon piggyBat from Myotis lucifugus is restricted by its own transposon ends," Nature Communications, Nature, vol. 16(1), pages 1-18, December.

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