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A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores

Author

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  • Alastair Skeffington

    (Max-Planck Institute of Molecular Plant Physiology
    University of Stirling)

  • Axel Fischer

    (Max-Planck Institute of Molecular Plant Physiology)

  • Sanja Sviben

    (Max-Planck Institute of Molecular Plant Physiology)

  • Magdalena Brzezinka

    (Max-Planck Institute of Molecular Plant Physiology)

  • Michał Górka

    (Max-Planck Institute of Molecular Plant Physiology)

  • Luca Bertinetti

    (Max Planck Institute of Colloids and Interfaces)

  • Christian Woehle

    (Max Planck-Genome-Centre Cologne)

  • Bruno Huettel

    (Max Planck-Genome-Centre Cologne)

  • Alexander Graf

    (Max-Planck Institute of Molecular Plant Physiology)

  • André Scheffel

    (Technische Universität Dresden, Faculty of Biology
    Max-Planck Institute of Molecular Plant Physiology)

Abstract

Coccolithophores are globally abundant, calcifying microalgae that have profound effects on marine biogeochemical cycles, the climate, and life in the oceans. They are characterized by a cell wall of CaCO3 scales called coccoliths, which may contribute to their ecological success. The intricate morphologies of coccoliths are of interest for biomimetic materials synthesis. Despite the global impact of coccolithophore calcification, we know little about the molecular machinery underpinning coccolithophore biology. Working on the model Emiliania huxleyi, a globally distributed bloom-former, we deploy a range of proteomic strategies to identify coccolithogenesis-related proteins. These analyses are supported by a new genome, with gene models derived from long-read transcriptome sequencing, which revealed many novel proteins specific to the calcifying haptophytes. Our experiments provide insights into proteins involved in various aspects of coccolithogenesis. Our improved genome, complemented with transcriptomic and proteomic data, constitutes a new resource for investigating fundamental aspects of coccolithophore biology.

Suggested Citation

  • Alastair Skeffington & Axel Fischer & Sanja Sviben & Magdalena Brzezinka & Michał Górka & Luca Bertinetti & Christian Woehle & Bruno Huettel & Alexander Graf & André Scheffel, 2023. "A joint proteomic and genomic investigation provides insights into the mechanism of calcification in coccolithophores," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39336-1
    DOI: 10.1038/s41467-023-39336-1
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    References listed on IDEAS

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    1. Karen Jiaxi Wang & Yongsong Huang & Markus Majaneva & Simon T. Belt & Sian Liao & Joseph Novak & Tyler R. Kartzinel & Timothy D. Herbert & Nora Richter & Patricia Cabedo-Sanz, 2021. "Group 2i Isochrysidales produce characteristic alkenones reflecting sea ice distribution," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    2. Sanja Sviben & Assaf Gal & Matthew A. Hood & Luca Bertinetti & Yael Politi & Mathieu Bennet & Praveen Krishnamoorthy & Andreas Schertel & Richard Wirth & Andrea Sorrentino & Eva Pereiro & Damien Faivr, 2016. "A vacuole-like compartment concentrates a disordered calcium phase in a key coccolithophorid alga," Nature Communications, Nature, vol. 7(1), pages 1-9, September.
    3. Amber L. Wells & Abel W. Lin & Li-Qiong Chen & Daniel Safer & Shane M. Cain & Tama Hasson & Bridget O. Carragher & Ronald A. Milligan & H. Lee Sweeney, 1999. "Myosin VI is an actin-based motor that moves backwards," Nature, Nature, vol. 401(6752), pages 505-508, September.
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