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Molecular mechanisms of Holliday junction branch migration catalyzed by an asymmetric RuvB hexamer

Author

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  • Anthony D. Rish

    (The Ohio State University
    The Ohio State University
    The Ohio State University Comprehensive Cancer Center)

  • Zhangfei Shen

    (The Ohio State University
    The Ohio State University Comprehensive Cancer Center)

  • Zhenhang Chen

    (The Ohio State University
    The Ohio State University Comprehensive Cancer Center
    Emory University School of Medicine)

  • Nan Zhang

    (The Ohio State University Comprehensive Cancer Center
    The Ohio State University)

  • Qingfei Zheng

    (The Ohio State University
    The Ohio State University
    The Ohio State University Comprehensive Cancer Center
    The Ohio State University)

  • Tian-Min Fu

    (The Ohio State University
    The Ohio State University
    The Ohio State University Comprehensive Cancer Center)

Abstract

The Holliday junction (HJ) is a DNA intermediate of homologous recombination, involved in many fundamental physiological processes. RuvB, an ATPase motor protein, drives branch migration of the Holliday junction with a mechanism that had yet to be elucidated. Here we report two cryo-EM structures of RuvB, providing a comprehensive understanding of HJ branch migration. RuvB assembles into a spiral staircase, ring-like hexamer, encircling dsDNA. Four protomers of RuvB contact the DNA backbone with a translocation step size of 2 nucleotides. The variation of nucleotide-binding states in RuvB supports a sequential model for ATP hydrolysis and nucleotide recycling, which occur at separate, singular positions. RuvB’s asymmetric assembly also explains the 6:4 stoichiometry between the RuvB/RuvA complex, which coordinates HJ migration in bacteria. Taken together, we provide a mechanistic understanding of HJ branch migration facilitated by RuvB, which may be universally shared by prokaryotic and eukaryotic organisms.

Suggested Citation

  • Anthony D. Rish & Zhangfei Shen & Zhenhang Chen & Nan Zhang & Qingfei Zheng & Tian-Min Fu, 2023. "Molecular mechanisms of Holliday junction branch migration catalyzed by an asymmetric RuvB hexamer," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39250-6
    DOI: 10.1038/s41467-023-39250-6
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    1. Andreas Martin & Tania A. Baker & Robert T. Sauer, 2005. "Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines," Nature, Nature, vol. 437(7062), pages 1115-1120, October.
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