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Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model

Author

Listed:
  • Zonghu Han

    (University of Minnesota)

  • Joseph Sushil Rao

    (University of Minnesota
    University of Minnesota)

  • Lakshya Gangwar

    (University of Minnesota)

  • Bat-Erdene Namsrai

    (University of Minnesota)

  • Jacqueline L. Pasek-Allen

    (University of Minnesota
    University of Minnesota)

  • Michael L. Etheridge

    (University of Minnesota)

  • Susan M. Wolf

    (University of Minnesota)

  • Timothy L. Pruett

    (University of Minnesota)

  • John C. Bischof

    (University of Minnesota
    University of Minnesota
    University of Minnesota)

  • Erik B. Finger

    (University of Minnesota)

Abstract

Banking cryopreserved organs could transform transplantation into a planned procedure that more equitably reaches patients regardless of geographical and time constraints. Previous organ cryopreservation attempts have failed primarily due to ice formation, but a promising alternative is vitrification, or the rapid cooling of organs to a stable, ice-free, glass-like state. However, rewarming of vitrified organs can similarly fail due to ice crystallization if rewarming is too slow or cracking from thermal stress if rewarming is not uniform. Here we use “nanowarming,” which employs alternating magnetic fields to heat nanoparticles within the organ vasculature, to achieve both rapid and uniform warming, after which the nanoparticles are removed by perfusion. We show that vitrified kidneys can be cryogenically stored (up to 100 days) and successfully recovered by nanowarming to allow transplantation and restore life-sustaining full renal function in nephrectomized recipients in a male rat model. Scaling this technology may one day enable organ banking for improved transplantation.

Suggested Citation

  • Zonghu Han & Joseph Sushil Rao & Lakshya Gangwar & Bat-Erdene Namsrai & Jacqueline L. Pasek-Allen & Michael L. Etheridge & Susan M. Wolf & Timothy L. Pruett & John C. Bischof & Erik B. Finger, 2023. "Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38824-8
    DOI: 10.1038/s41467-023-38824-8
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    References listed on IDEAS

    as
    1. David Nasralla & Constantin C. Coussios & Hynek Mergental & M. Zeeshan Akhtar & Andrew J. Butler & Carlo D. L. Ceresa & Virginia Chiocchia & Susan J. Dutton & Juan Carlos García-Valdecasas & Nigel Hea, 2018. "A randomized trial of normothermic preservation in liver transplantation," Nature, Nature, vol. 557(7703), pages 50-56, May.
    2. Shannon N. Tessier & Reinier J. de Vries & Casie A. Pendexter & Stephanie E. J. Cronin & Sinan Ozer & Ehab O. A. Hafiz & Siavash Raigani & Joao Paulo Oliveira-Costa & Benjamin T. Wilks & Manuela Loper, 2022. "Partial freezing of rat livers extends preservation time by 5-fold," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
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