IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37660-0.html
   My bibliography  Save this article

Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization

Author

Listed:
  • Xin Huang

    (Cellular and Developmental Biology, Yale University
    Yale University)

  • Thomas Nero

    (Cellular and Developmental Biology, Yale University)

  • Ranjuna Weerasekera

    (Molecular Biophysics Program, Wesleyan University)

  • Katherine H. Matej

    (Cellular and Developmental Biology, Yale University)

  • Alex Hinbest

    (Molecular Biophysics Program, Wesleyan University)

  • Zhaowei Jiang

    (Cellular and Developmental Biology, Yale University)

  • Rebecca F. Lee

    (Yale School of Medicine)

  • Longjun Wu

    (Yale University
    The Hong Kong University of Science and Technology, Hong Kong SAR)

  • Cecilia Chak

    (Cellular and Developmental Biology, Yale University)

  • Japinder Nijjer

    (Cellular and Developmental Biology, Yale University)

  • Isabella Gibaldi

    (Molecular Biophysics Program, Wesleyan University)

  • Hang Yang

    (Molecular Biophysics Program, Wesleyan University)

  • Nathan Gamble

    (Molecular Biophysics Program, Wesleyan University)

  • Wai-Leung Ng

    (Tufts University School of Medicine)

  • Stacy A. Malaker

    (Yale University)

  • Kaelyn Sumigray

    (Yale School of Medicine
    Yale Stem Cell Center, Yale School of Medicine
    Yale Cancer Center, Yale School of Medicine)

  • Rich Olson

    (Molecular Biophysics Program, Wesleyan University)

  • Jing Yan

    (Cellular and Developmental Biology, Yale University
    Yale University)

Abstract

Bacterial biofilms are formed on environmental surfaces and host tissues, and facilitate host colonization and antibiotic resistance by human pathogens. Bacteria often express multiple adhesive proteins (adhesins), but it is often unclear whether adhesins have specialized or redundant roles. Here, we show how the model biofilm-forming organism Vibrio cholerae uses two adhesins with overlapping but distinct functions to achieve robust adhesion to diverse surfaces. Both biofilm-specific adhesins Bap1 and RbmC function as a “double-sided tape”: they share a β-propeller domain that binds to the biofilm matrix exopolysaccharide, but have distinct environment-facing domains. Bap1 adheres to lipids and abiotic surfaces, while RbmC mainly mediates binding to host surfaces. Furthermore, both adhesins contribute to adhesion in an enteroid monolayer colonization model. We expect that similar modular domains may be utilized by other pathogens, and this line of research can potentially lead to new biofilm-removal strategies and biofilm-inspired adhesives.

Suggested Citation

  • Xin Huang & Thomas Nero & Ranjuna Weerasekera & Katherine H. Matej & Alex Hinbest & Zhaowei Jiang & Rebecca F. Lee & Longjun Wu & Cecilia Chak & Japinder Nijjer & Isabella Gibaldi & Hang Yang & Nathan, 2023. "Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37660-0
    DOI: 10.1038/s41467-023-37660-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37660-0
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-37660-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Thomas J. Kirn & Brooke A. Jude & Ronald K. Taylor, 2005. "A colonization factor links Vibrio cholerae environmental survival and human infection," Nature, Nature, vol. 438(7069), pages 863-866, December.
    2. Kyle A. Floyd & Calvin K. Lee & Wujing Xian & Mahmoud Nametalla & Aneesa Valentine & Benjamin Crair & Shiwei Zhu & Hannah Q. Hughes & Jennifer L. Chlebek & Daniel C. Wu & Jin Hwan Park & Ali M. Farhat, 2020. "c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Cong Liu & Di Sun & Jiawen Liu & Ying Chen & Xuge Zhou & Yunrui Ru & Jingrong Zhu & Weijie Liu, 2022. "cAMP and c-di-GMP synergistically support biofilm maintenance through the direct interaction of their effectors," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Gareth J Williams & Greta S Aeby & Rebecca O M Cowie & Simon K Davy, 2010. "Predictive Modeling of Coral Disease Distribution within a Reef System," PLOS ONE, Public Library of Science, vol. 5(2), pages 1-10, February.
    3. Xia Li & Wenfang Yin & Junjie Desmond Lin & Yong Zhang & Quan Guo & Gerun Wang & Xiayu Chen & Binbin Cui & Mingfang Wang & Min Chen & Peng Li & Ya-Wen He & Wei Qian & Haibin Luo & Lian-Hui Zhang & Xue, 2023. "Regulation of the physiology and virulence of Ralstonia solanacearum by the second messenger 2′,3′-cyclic guanosine monophosphate," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    4. María Pérez-Burgos & Marco Herfurth & Andreas Kaczmarczyk & Andrea Harms & Katrin Huber & Urs Jenal & Timo Glatter & Lotte Søgaard-Andersen, 2024. "A deterministic, c-di-GMP-dependent program ensures the generation of phenotypically similar, symmetric daughter cells during cytokinesis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37660-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.