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Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization

Author

Listed:
  • Xin Huang

    (Cellular and Developmental Biology, Yale University
    Yale University)

  • Thomas Nero

    (Cellular and Developmental Biology, Yale University)

  • Ranjuna Weerasekera

    (Molecular Biophysics Program, Wesleyan University)

  • Katherine H. Matej

    (Cellular and Developmental Biology, Yale University)

  • Alex Hinbest

    (Molecular Biophysics Program, Wesleyan University)

  • Zhaowei Jiang

    (Cellular and Developmental Biology, Yale University)

  • Rebecca F. Lee

    (Yale School of Medicine)

  • Longjun Wu

    (Yale University
    The Hong Kong University of Science and Technology, Hong Kong SAR)

  • Cecilia Chak

    (Cellular and Developmental Biology, Yale University)

  • Japinder Nijjer

    (Cellular and Developmental Biology, Yale University)

  • Isabella Gibaldi

    (Molecular Biophysics Program, Wesleyan University)

  • Hang Yang

    (Molecular Biophysics Program, Wesleyan University)

  • Nathan Gamble

    (Molecular Biophysics Program, Wesleyan University)

  • Wai-Leung Ng

    (Tufts University School of Medicine)

  • Stacy A. Malaker

    (Yale University)

  • Kaelyn Sumigray

    (Yale School of Medicine
    Yale Stem Cell Center, Yale School of Medicine
    Yale Cancer Center, Yale School of Medicine)

  • Rich Olson

    (Molecular Biophysics Program, Wesleyan University)

  • Jing Yan

    (Cellular and Developmental Biology, Yale University
    Yale University)

Abstract

Bacterial biofilms are formed on environmental surfaces and host tissues, and facilitate host colonization and antibiotic resistance by human pathogens. Bacteria often express multiple adhesive proteins (adhesins), but it is often unclear whether adhesins have specialized or redundant roles. Here, we show how the model biofilm-forming organism Vibrio cholerae uses two adhesins with overlapping but distinct functions to achieve robust adhesion to diverse surfaces. Both biofilm-specific adhesins Bap1 and RbmC function as a “double-sided tape”: they share a β-propeller domain that binds to the biofilm matrix exopolysaccharide, but have distinct environment-facing domains. Bap1 adheres to lipids and abiotic surfaces, while RbmC mainly mediates binding to host surfaces. Furthermore, both adhesins contribute to adhesion in an enteroid monolayer colonization model. We expect that similar modular domains may be utilized by other pathogens, and this line of research can potentially lead to new biofilm-removal strategies and biofilm-inspired adhesives.

Suggested Citation

  • Xin Huang & Thomas Nero & Ranjuna Weerasekera & Katherine H. Matej & Alex Hinbest & Zhaowei Jiang & Rebecca F. Lee & Longjun Wu & Cecilia Chak & Japinder Nijjer & Isabella Gibaldi & Hang Yang & Nathan, 2023. "Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37660-0
    DOI: 10.1038/s41467-023-37660-0
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    References listed on IDEAS

    as
    1. Thomas J. Kirn & Brooke A. Jude & Ronald K. Taylor, 2005. "A colonization factor links Vibrio cholerae environmental survival and human infection," Nature, Nature, vol. 438(7069), pages 863-866, December.
    2. Kyle A. Floyd & Calvin K. Lee & Wujing Xian & Mahmoud Nametalla & Aneesa Valentine & Benjamin Crair & Shiwei Zhu & Hannah Q. Hughes & Jennifer L. Chlebek & Daniel C. Wu & Jin Hwan Park & Ali M. Farhat, 2020. "c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
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