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An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice

Author

Listed:
  • Sverre Grødem

    (University of Oslo)

  • Ingeborg Nymoen

    (University of Oslo)

  • Guro Helén Vatne

    (University of Oslo)

  • Frederik Sebastian Rogge

    (University of Oslo)

  • Valgerður Björnsdóttir

    (University of Oslo)

  • Kristian Kinden Lensjø

    (University of Oslo)

  • Marianne Fyhn

    (University of Oslo)

Abstract

Genetically encoded Ca2+ indicators (GECIs) are widely used to measure neural activity. Here, we explore the use of systemically administered PHP.eB AAVs for brain-wide expression of GECIs and compare the expression properties to intracerebrally injected AAVs in male mice. We show that systemic administration is a promising strategy for imaging neural activity. Next, we establish the use of EE-RR- (soma) and RPL10a (Ribo) soma-targeting peptides with the latest jGCaMP and show that EE-RR-tagged jGCaMP8 gives rise to strong expression but limited soma-targeting. In contrast, Ribo-tagged jGCaMP8 lacks neuropil signal, but the expression rate is reduced. To combat this, we modified the linker region of the Ribo-tag (RiboL1-). RiboL1-jGCaMP8 expresses faster than Ribo-jGCaMP8 but remains too dim for reliable use with systemic virus administration. However, intracerebral injections of the RiboL1-tagged jGCaMP8 constructs provide strong Ca2+ signals devoid of neuropil contamination, with remarkable labeling density.

Suggested Citation

  • Sverre Grødem & Ingeborg Nymoen & Guro Helén Vatne & Frederik Sebastian Rogge & Valgerður Björnsdóttir & Kristian Kinden Lensjø & Marianne Fyhn, 2023. "An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36324-3
    DOI: 10.1038/s41467-023-36324-3
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    References listed on IDEAS

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    1. Yaxiong Yang & Nan Liu & Yuanyuan He & Yuxia Liu & Lin Ge & Linzhi Zou & Sen Song & Wei Xiong & Xiaodong Liu, 2018. "Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP," Nature Communications, Nature, vol. 9(1), pages 1-18, December.
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