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Jag1-Notch cis-interaction determines cell fate segregation in pancreatic development

Author

Listed:
  • Xiaochan Xu

    (University of Copenhagen)

  • Philip Allan Seymour

    (University of Copenhagen
    University of Copenhagen)

  • Kim Sneppen

    (University of Copenhagen)

  • Ala Trusina

    (University of Copenhagen)

  • Anuska la Rosa Egeskov-Madsen

    (University of Copenhagen
    University of Copenhagen)

  • Mette Christine Jørgensen

    (University of Copenhagen
    University of Copenhagen)

  • Mogens Høgh Jensen

    (University of Copenhagen)

  • Palle Serup

    (University of Copenhagen
    University of Copenhagen)

Abstract

The Notch ligands Jag1 and Dll1 guide differentiation of multipotent pancreatic progenitor cells (MPCs) into unipotent pro-acinar cells (PACs) and bipotent duct/endocrine progenitors (BPs). Ligand-mediated trans-activation of Notch receptors induces oscillating expression of the transcription factor Hes1, while ligand-receptor cis-interaction indirectly represses Hes1 activation. Despite Dll1 and Jag1 both displaying cis- and trans-interactions, the two mutants have different phenotypes for reasons not fully understood. Here, we present a mathematical model that recapitulates the spatiotemporal differentiation of MPCs into PACs and BPs. The model correctly captures cell fate changes in Notch pathway knockout mice and small molecule inhibitor studies, and a requirement for oscillatory Hes1 expression to maintain the multipotent state. Crucially, the model entails cell-autonomous attenuation of Notch signaling by Jag1-mediated cis-inhibition in MPC differentiation. The model sheds light on the underlying mechanisms, suggesting that cis-interaction is crucial for exiting the multipotent state, while trans-interaction is required for adopting the bipotent fate.

Suggested Citation

  • Xiaochan Xu & Philip Allan Seymour & Kim Sneppen & Ala Trusina & Anuska la Rosa Egeskov-Madsen & Mette Christine Jørgensen & Mogens Høgh Jensen & Palle Serup, 2023. "Jag1-Notch cis-interaction determines cell fate segregation in pancreatic development," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-35963-w
    DOI: 10.1038/s41467-023-35963-w
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    References listed on IDEAS

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