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An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing

Author

Listed:
  • Feng Xiong

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Chinese Academy of Sciences)

  • Hong Yang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yi-Ge Song

    (Huazhong University of Science and Technology)

  • Hai-Bin Qin

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Qing-Yang Zhang

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xian Huang

    (Huazhong University of Science and Technology)

  • Wei Jing

    (Huazhong University of Science and Technology)

  • Manfei Deng

    (Huazhong University of Science and Technology)

  • Yang Liu

    (National Institute of Biological Sciences)

  • Zhixiang Liu

    (National Institute of Biological Sciences)

  • Yin Shen

    (Wuhan University)

  • Yunyun Han

    (Wuhan University)

  • Youming Lu

    (Huazhong University of Science and Technology)

  • Xiangmin Xu

    (University of California
    University of California)

  • Todd C. Holmes

    (University of California
    University of California)

  • Minmin Luo

    (National Institute of Biological Sciences
    Chinese Institute for Brain Research)

  • Fei Zhao

    (Chinese Institute for Brain Research
    Capital Medical University)

  • Min-Hua Luo

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences
    Chinese Academy of Sciences
    University of California)

  • Wen-Bo Zeng

    (Chinese Academy of Sciences)

Abstract

Monosynaptic viral tracers are essential tools for dissecting neuronal connectomes and for targeted delivery of molecular sensors and effectors. Viral toxicity and complex multi-injection protocols are major limiting application barriers. To overcome these barriers, we developed an anterograde monosynaptic H129Amp tracer system based on HSV-1 strain H129. The H129Amp tracer system consists of two components: an H129-dTK-T2-pacFlox helper which assists H129Amp tracer’s propagation and transneuronal monosynaptic transmission. The shared viral features of tracer/helper allow for simultaneous single-injection and subsequent high expression efficiency from multiple-copy of expression cassettes in H129Amp tracer. These improvements of H129Amp tracer system shorten experiment duration from 28-day to 5-day for fast-bright monosynaptic tracing. The lack of toxic viral genes in the H129Amp tracer minimizes toxicity in postsynaptic neurons, thus offering the potential for functional anterograde mapping and long-term tracer delivery of genetic payloads. The H129Amp tracer system is a powerful tracing tool for revealing neuronal connectomes.

Suggested Citation

  • Feng Xiong & Hong Yang & Yi-Ge Song & Hai-Bin Qin & Qing-Yang Zhang & Xian Huang & Wei Jing & Manfei Deng & Yang Liu & Zhixiang Liu & Yin Shen & Yunyun Han & Youming Lu & Xiangmin Xu & Todd C. Holmes , 2022. "An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35355-6
    DOI: 10.1038/s41467-022-35355-6
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    1. Lindsay A. Schwarz & Kazunari Miyamichi & Xiaojing J. Gao & Kevin T. Beier & Brandon Weissbourd & Katherine E. DeLoach & Jing Ren & Sandy Ibanes & Robert C. Malenka & Eric J. Kremer & Liqun Luo, 2015. "Viral-genetic tracing of the input–output organization of a central noradrenaline circuit," Nature, Nature, vol. 524(7563), pages 88-92, August.
    2. Houze Zhu & Huanhuan Yan & Na Tang & Xinyan Li & Pei Pang & Hao Li & Wenting Chen & Yu Guo & Shu Shu & You Cai & Lei Pei & Dan Liu & Min-Hua Luo & Hengye Man & Qing Tian & Yangling Mu & Ling-Qiang Zhu, 2017. "Impairments of spatial memory in an Alzheimer’s disease model via degeneration of hippocampal cholinergic synapses," Nature Communications, Nature, vol. 8(1), pages 1-13, December.
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