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Acousto-holographic reconstruction of whole-cell stiffness maps

Author

Listed:
  • Rahmetullah Varol

    (Yildiz Technical University
    Universität der Bundeswehr München)

  • Zeynep Karavelioglu

    (Yildiz Technical University
    Paul Scherrer Institute
    Eidgenössische Technische Hochschule Zürich)

  • Sevde Omeroglu

    (Gebze Technical University)

  • Gizem Aydemir

    (Yildiz Technical University
    Eidgenössische Technische Hochschule Zürich)

  • Aslihan Karadag

    (Dokuz Eylul University)

  • Hanife E. Meco

    (Dokuz Eylul University)

  • Ali A. Demircali

    (Yildiz Technical University
    Hamlyn Centre, Imperial College London)

  • Abdurrahim Yilmaz

    (Yildiz Technical University)

  • Gizem C. Kocal

    (Dokuz Eylul University)

  • Gulsum Gencoglan

    (Istinye University)

  • Muhammed E. Oruc

    (Gebze Technical University)

  • Gokhan B. Esmer

    (Marmara University)

  • Yasemin Basbinar

    (Dokuz Eylul University)

  • Sahin K. Ozdemir

    (The Pennsylvania State University)

  • Huseyin Uvet

    (Yildiz Technical University)

Abstract

Accurate assessment of cell stiffness distribution is essential due to the critical role of cell mechanobiology in regulation of vital cellular processes like proliferation, adhesion, migration, and motility. Stiffness provides critical information in understanding onset and progress of various diseases, including metastasis and differentiation of cancer. Atomic force microscopy and optical trapping set the gold standard in stiffness measurements. However, their widespread use has been hampered with long processing times, unreliable contact point determination, physical damage to cells, and unsuitability for multiple cell analysis. Here, we demonstrate a simple, fast, label-free, and high-resolution technique using acoustic stimulation and holographic imaging to reconstruct stiffness maps of single cells. We used this acousto-holographic method to determine stiffness maps of HCT116 and CTC-mimicking HCT116 cells and differentiate between them. Our system would enable widespread use of whole-cell stiffness measurements in clinical and research settings for cancer studies, disease modeling, drug testing, and diagnostics.

Suggested Citation

  • Rahmetullah Varol & Zeynep Karavelioglu & Sevde Omeroglu & Gizem Aydemir & Aslihan Karadag & Hanife E. Meco & Ali A. Demircali & Abdurrahim Yilmaz & Gizem C. Kocal & Gulsum Gencoglan & Muhammed E. Oru, 2022. "Acousto-holographic reconstruction of whole-cell stiffness maps," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35075-x
    DOI: 10.1038/s41467-022-35075-x
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    References listed on IDEAS

    as
    1. Benjamin L. Bangasser & Ghaidan A. Shamsan & Clarence E. Chan & Kwaku N. Opoku & Erkan Tüzel & Benjamin W. Schlichtmann & Jesse A. Kasim & Benjamin J. Fuller & Brannon R. McCullough & Steven S. Rosenf, 2017. "Shifting the optimal stiffness for cell migration," Nature Communications, Nature, vol. 8(1), pages 1-10, August.
    2. Molly A. May & Nicolas Barré & Kai K. Kummer & Michaela Kress & Monika Ritsch-Marte & Alexander Jesacher, 2021. "Fast holographic scattering compensation for deep tissue biological imaging," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
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