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The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane

Author

Listed:
  • Claudia Matthaeus

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Kem A. Sochacki

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Andrea M. Dickey

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

  • Dmytro Puchkov

    (Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP))

  • Volker Haucke

    (Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
    Faculty of Biology, Chemistry and Pharmacy, Freie Universität Berlin)

  • Martin Lehmann

    (Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP))

  • Justin W. Taraska

    (National Heart, Lung, and Blood Institute, National Institutes of Health)

Abstract

Caveolae are small coated plasma membrane invaginations with diverse functions. Caveolae undergo curvature changes. Yet, it is unclear which proteins regulate this process. To address this gap, we develop a correlative stimulated emission depletion (STED) fluorescence and platinum replica electron microscopy imaging (CLEM) method to image proteins at single caveolae. Caveolins and cavins are found at all caveolae, independent of curvature. EHD2 is detected at both low and highly curved caveolae. Pacsin2 associates with low curved caveolae and EHBP1 with mostly highly curved caveolae. Dynamin is absent from caveolae. Cells lacking dynamin show no substantial changes to caveolae, suggesting that dynamin is not directly involved in caveolae curvature. We propose a model where caveolins, cavins, and EHD2 assemble as a cohesive structural unit regulated by intermittent associations with pacsin2 and EHBP1. These coats can flatten and curve to enable lipid traffic, signaling, and changes to the surface area of the cell.

Suggested Citation

  • Claudia Matthaeus & Kem A. Sochacki & Andrea M. Dickey & Dmytro Puchkov & Volker Haucke & Martin Lehmann & Justin W. Taraska, 2022. "The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34958-3
    DOI: 10.1038/s41467-022-34958-3
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    References listed on IDEAS

    as
    1. Melissa Dewulf & Darius Vasco Köster & Bidisha Sinha & Christine Viaris de Lesegno & Valérie Chambon & Anne Bigot & Mona Bensalah & Elisa Negroni & Nicolas Tardif & Joanna Podkalicka & Ludger Johannes, 2019. "Dystrophy-associated caveolin-3 mutations reveal that caveolae couple IL6/STAT3 signaling with mechanosensing in human muscle cells," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
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    Cited by:

    1. Fabian Lukas & Claudia Matthaeus & Tania López-Hernández & Ines Lahmann & Nicole Schultz & Martin Lehmann & Dmytro Puchkov & Jan Pielage & Volker Haucke & Tanja Maritzen, 2024. "Canonical and non-canonical integrin-based adhesions dynamically interconvert," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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