IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-34941-y.html
   My bibliography  Save this article

Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics

Author

Listed:
  • Patrick Vogel

    (Julius-Maximilians-University Würzburg)

  • Martin Andreas Rückert

    (Julius-Maximilians-University Würzburg)

  • Bernhard Friedrich

    (University Hospital Erlangen)

  • Rainer Tietze

    (University Hospital Erlangen)

  • Stefan Lyer

    (University Hospital Erlangen)

  • Thomas Kampf

    (Julius-Maximilians-University Würzburg
    University Hospital Würzburg)

  • Thomas Hennig

    (Julius-Maximilians-University Würzburg)

  • Lars Dölken

    (Julius-Maximilians-University Würzburg
    Helmholtz-Center for Infection Research)

  • Christoph Alexiou

    (University Hospital Erlangen)

  • Volker Christian Behr

    (Julius-Maximilians-University Würzburg)

Abstract

Magnetic nanoparticles (MNPs) have been adapted for many applications, e.g., bioassays for the detection of biomarkers such as antibodies, by controlled engineering of specific surface properties. Specific measurement of such binding states is of high interest but currently limited to highly sensitive techniques such as ELISA or flow cytometry, which are relatively inflexible, difficult to handle, expensive and time-consuming. Here we report a method named COMPASS (Critical-Offset-Magnetic-Particle-SpectroScopy), which is based on a critical offset magnetic field, enabling sensitive detection to minimal changes in mobility of MNP ensembles, e.g., resulting from SARS-CoV-2 antibodies binding to the S antigen on the surface of functionalized MNPs. With a sensitivity of 0.33 fmole/50 µl (≙7 pM) for SARS-CoV-2-S1 antibodies, measured with a low-cost portable COMPASS device, the proposed technique is competitive with respect to sensitivity while providing flexibility, robustness, and a measurement time of seconds per sample. In addition, initial results with blood serum demonstrate high specificity.

Suggested Citation

  • Patrick Vogel & Martin Andreas Rückert & Bernhard Friedrich & Rainer Tietze & Stefan Lyer & Thomas Kampf & Thomas Hennig & Lars Dölken & Christoph Alexiou & Volker Christian Behr, 2022. "Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34941-y
    DOI: 10.1038/s41467-022-34941-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-34941-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-34941-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34941-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.