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Altered tRNA processing is linked to a distinct and unusual La protein in Tetrahymena thermophila

Author

Listed:
  • Kyra Kerkhofs

    (York University)

  • Jyoti Garg

    (York University)

  • Étienne Fafard-Couture

    (Université de Sherbrooke)

  • Sherif Abou Elela

    (Université de Sherbrooke)

  • Michelle S. Scott

    (Université de Sherbrooke)

  • Ronald E. Pearlman

    (York University)

  • Mark A. Bayfield

    (York University)

Abstract

Nascent pre-tRNAs are transcribed by RNA polymerase III and immediately bound by La proteins on the UUU-3’OH sequence, using a tandem arrangement of the La motif and an adjacent RNA recognition motif-1 (RRM1), resulting in protection from 3’-exonucleases and promotion of pre-tRNA folding. The Tetrahymena thermophila protein Mlp1 has been previously classified as a genuine La protein, despite the predicted absence of the RRM1. We find that Mlp1 functions as a La protein through binding of pre-tRNAs, and affects pre-tRNA processing in Tetrahymena thermophila and when expressed in fission yeast. However, unlike in other examined eukaryotes, depletion of Mlp1 results in 3’-trailer stabilization. The 3’-trailers in Tetrahymena thermophila are uniquely short relative to other examined eukaryotes, and 5’-leaders have evolved to disfavour pre-tRNA leader/trailer pairing. Our data indicate that this variant Mlp1 architecture is linked to an altered, novel mechanism of tRNA processing in Tetrahymena thermophila.

Suggested Citation

  • Kyra Kerkhofs & Jyoti Garg & Étienne Fafard-Couture & Sherif Abou Elela & Michelle S. Scott & Ronald E. Pearlman & Mark A. Bayfield, 2022. "Altered tRNA processing is linked to a distinct and unusual La protein in Tetrahymena thermophila," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34796-3
    DOI: 10.1038/s41467-022-34796-3
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    1. Laura L. Arthur & Joyce J. Chung & Preetam Janakirama & Kathryn M. Keefer & Igor Kolotilin & Slavica Pavlovic-Djuranovic & Douglas L. Chalker & Vojislava Grbic & Rachel Green & Rima Menassa & Heather , 2017. "Rapid generation of hypomorphic mutations," Nature Communications, Nature, vol. 8(1), pages 1-16, April.
    2. Laura L. Arthur & Joyce J. Chung & Preetam Janakirama & Kathryn M. Keefer & Igor Kolotilin & Slavica Pavlovic-Djuranovic & Douglas L. Chalker & Vojislava Grbic & Rachel Green & Rima Menassa & Heather , 2017. "Correction: Corrigendum: Rapid generation of hypomorphic mutations," Nature Communications, Nature, vol. 8(1), pages 1-1, April.
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