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Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre

Author

Listed:
  • Katie J. Harding

    (University of California
    Lawrence Livermore National Laboratory)

  • Kendra A. Turk-Kubo

    (University of California)

  • Esther Wing Kwan Mak

    (University of California)

  • Peter K. Weber

    (Lawrence Livermore National Laboratory)

  • Xavier Mayali

    (Lawrence Livermore National Laboratory)

  • Jonathan P. Zehr

    (University of California)

Abstract

Biological nitrogen fixation is a major important source of nitrogen for low-nutrient surface oceanic waters. Nitrogen-fixing (diazotrophic) cyanobacteria are believed to be the primary contributors to this process, but the contribution of non-cyanobacterial diazotrophic organisms in oxygenated surface water, while hypothesized to be important, has yet to be demonstrated. In this study, we used simultaneous 15N-dinitrogen and 13C-bicarbonate incubations combined with nanoscale secondary ion mass spectrometry analysis to screen tens of thousands of mostly particle-associated, cell-like regions of interest collected from the North Pacific Subtropical Gyre. These dual isotope incubations allow us to distinguish between non-cyanobacterial and cyanobacterial nitrogen-fixing microorganisms and to measure putative cell-specific nitrogen fixation rates. With this approach, we detect nitrogen fixation by putative non-cyanobacterial diazotrophs in the oxygenated surface ocean, which are associated with organic-rich particles (

Suggested Citation

  • Katie J. Harding & Kendra A. Turk-Kubo & Esther Wing Kwan Mak & Peter K. Weber & Xavier Mayali & Jonathan P. Zehr, 2022. "Cell-specific measurements show nitrogen fixation by particle-attached putative non-cyanobacterial diazotrophs in the North Pacific Subtropical Gyre," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34585-y
    DOI: 10.1038/s41467-022-34585-y
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    References listed on IDEAS

    as
    1. Christopher R. Schvarcz & Samuel T. Wilson & Mathieu Caffin & Rosalina Stancheva & Qian Li & Kendra A. Turk-Kubo & Angelicque E. White & David M. Karl & Jonathan P. Zehr & Grieg F. Steward, 2022. "Overlooked and widespread pennate diatom-diazotroph symbioses in the sea," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Juan José Pierella Karlusich & Eric Pelletier & Fabien Lombard & Madeline Carsique & Etienne Dvorak & Sébastien Colin & Marc Picheral & Francisco M. Cornejo-Castillo & Silvia G. Acinas & Rainer Pepper, 2021. "Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods," Nature Communications, Nature, vol. 12(1), pages 1-18, December.
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