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Functionalized graphene grids with various charges for single-particle cryo-EM

Author

Listed:
  • Ye Lu

    (Tsinghua University
    Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Nan Liu

    (Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Yongbo Liu

    (Tsinghua University
    Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Liming Zheng

    (Peking University
    Beijing Graphene Institute)

  • Junhao Yang

    (Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Jia Wang

    (Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Xia Jia

    (Tsinghua University
    Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Qinru Zi

    (Tsinghua University
    Wuhan University)

  • Hailin Peng

    (Peking University
    Beijing Graphene Institute)

  • Yu Rao

    (Tsinghua University
    Tsinghua University
    Tsinghua University
    Tsinghua University)

  • Hong-Wei Wang

    (Tsinghua University
    Tsinghua University
    Tsinghua University
    Tsinghua University)

Abstract

A major hurdle for single particle cryo-EM in structural determination lies in the specimen preparation impaired by the air-water interface (AWI) and preferential particle-orientation problems. In this work, we develop functionalized graphene grids with various charges via a dediazoniation reaction for cryo-EM specimen preparation. The graphene grids are paraffin-assistant fabricated, which appear with less contaminations compared with those produced by polymer transfer method. By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle-orientation distribution for more robust single particle cryo-EM structural determination.

Suggested Citation

  • Ye Lu & Nan Liu & Yongbo Liu & Liming Zheng & Junhao Yang & Jia Wang & Xia Jia & Qinru Zi & Hailin Peng & Yu Rao & Hong-Wei Wang, 2022. "Functionalized graphene grids with various charges for single-particle cryo-EM," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34579-w
    DOI: 10.1038/s41467-022-34579-w
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    References listed on IDEAS

    as
    1. Liming Zheng & Yanan Chen & Ning Li & Jincan Zhang & Nan Liu & Junjie Liu & Wenhui Dang & Bing Deng & Yanbin Li & Xiaoyin Gao & Congwei Tan & Zi Yang & Shipu Xu & Mingzhan Wang & Hao Yang & Luzhao Sun, 2020. "Robust ultraclean atomically thin membranes for atomic-resolution electron microscopy," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
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    Cited by:

    1. Kaitlyn M. Abe & Gan Li & Qixiang He & Timothy Grant & Ci Ji Lim, 2024. "Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Un Seng Chio & Eugene Palovcak & Anton A. A. Smith & Henriette Autzen & Elise N. Muñoz & Zanlin Yu & Feng Wang & David A. Agard & Jean-Paul Armache & Geeta J. Narlikar & Yifan Cheng, 2024. "Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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