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Spheroplasts preparation boosts the catalytic potential of a squalene-hopene cyclase

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Listed:
  • Ana I. Benítez-Mateos

    (University of Bern)

  • Andreas Schneider

    (University of Stuttgart)

  • Eimear Hegarty

    (University of Bern)

  • Bernhard Hauer

    (University of Stuttgart)

  • Francesca Paradisi

    (University of Bern)

Abstract

Squalene-hopene cyclases are a highly valuable and attractive class of membrane-bound enzymes as sustainable biotechnological tools to produce aromas and bioactive compounds at industrial scale. However, their application as whole-cell biocatalysts suffer from the outer cell membrane acting as a diffusion barrier for the highly hydrophobic substrate/product, while the use of purified enzymes leads to dramatic loss of stability. Here we present an unexplored strategy for biocatalysis: the application of squalene-hopene-cyclase spheroplasts. By removing the outer cell membrane, we produce stable and substrate-accessible biocatalysts. These spheroplasts exhibit up to 100-fold higher activity than their whole-cell counterparts for the biotransformations of squalene, geranyl acetone, farnesol, and farnesyl acetone. Their catalytic ability is also higher than the purified enzyme for all high molecular weight terpenes. In addition, we introduce a concept for the carrier-free immobilization of spheroplasts via crosslinking, crosslinked spheroplasts. The crosslinked spheroplasts maintain the same catalytic activity of the spheroplasts, offering additional advantages such as recycling and reuse. These timely solutions contribute not only to harness the catalytic potential of the squalene-hopene cyclases, but also to make biocatalytic processes even greener and more cost-efficient.

Suggested Citation

  • Ana I. Benítez-Mateos & Andreas Schneider & Eimear Hegarty & Bernhard Hauer & Francesca Paradisi, 2022. "Spheroplasts preparation boosts the catalytic potential of a squalene-hopene cyclase," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34030-0
    DOI: 10.1038/s41467-022-34030-0
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    Cited by:

    1. Andreas Schneider & Thomas B. Lystbæk & Daniel Markthaler & Niels Hansen & Bernhard Hauer, 2024. "Biocatalytic stereocontrolled head-to-tail cyclizations of unbiased terpenes as a tool in chemoenzymatic synthesis," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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