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Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors

Author

Listed:
  • Hucheng Chang

    (University of Natural Resources and Life Sciences)

  • Neus Gacias Amengual

    (University of Natural Resources and Life Sciences)

  • Alexander Botz

    (University of Natural Resources and Life Sciences)

  • Lorenz Schwaiger

    (University of Natural Resources and Life Sciences)

  • Daniel Kracher

    (University of Natural Resources and Life Sciences
    Graz University of Technology)

  • Stefan Scheiblbrandner

    (University of Natural Resources and Life Sciences)

  • Florian Csarman

    (University of Natural Resources and Life Sciences)

  • Roland Ludwig

    (University of Natural Resources and Life Sciences)

Abstract

Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing saccharification efficiency and rate. Recent studies demonstrate that H2O2 rather than O2 is the cosubstrate of the LPMO-catalyzed depolymerization of polysaccharides. Some studies have questioned the physiological relevance of the H2O2-based mechanism for plant cell wall degradation. This study reports the localized and time-resolved determination of LPMO activity on poplar wood cell walls by measuring the H2O2 concentration in their vicinity with a piezo-controlled H2O2 microsensor. The investigated Neurospora crassa LPMO binds to the inner cell wall layer and consumes enzymatically generated H2O2. The results point towards a high catalytic efficiency of LPMO at a low H2O2 concentration that auxiliary oxidoreductases in fungal secretomes can easily generate. Measurements with a glucose microbiosensor additionally demonstrate that LPMO promotes cellobiohydrolase activity on wood cell walls and plays a synergistic role in the fungal extracellular catabolism and in industrial biomass degradation.

Suggested Citation

  • Hucheng Chang & Neus Gacias Amengual & Alexander Botz & Lorenz Schwaiger & Daniel Kracher & Stefan Scheiblbrandner & Florian Csarman & Roland Ludwig, 2022. "Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33963-w
    DOI: 10.1038/s41467-022-33963-w
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    References listed on IDEAS

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    1. Leila Lo Leggio & Thomas J. Simmons & Jens-Christian N. Poulsen & Kristian E. H. Frandsen & Glyn R. Hemsworth & Mary A. Stringer & Pernille von Freiesleben & Morten Tovborg & Katja S. Johansen & Leona, 2015. "Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    2. Riin Kont & Bastien Bissaro & Vincent G. H. Eijsink & Priit Väljamäe, 2020. "Kinetic insights into the peroxygenase activity of cellulose-active lytic polysaccharide monooxygenases (LPMOs)," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
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    Cited by:

    1. Iván Ayuso-Fernández & Tom Z. Emrich-Mills & Julia Haak & Ole Golten & Kelsi R. Hall & Lorenz Schwaiger & Trond S. Moe & Anton A. Stepnov & Roland Ludwig & George E. Cutsail III & Morten Sørlie & Åsmu, 2024. "Mutational dissection of a hole hopping route in a lytic polysaccharide monooxygenase (LPMO)," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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